SRP6448
Fas Ligand/FasL human
recombinant, expressed in HEK 293 cells, ≥95% (SDS-PAGE)
别名:
ALPS1B, APT1LG1, CD178, CD95-L, CD95L, FASL, FASLG, Fas ligand, TNFSF6
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关于此项目
NACRES:
NA.32
UNSPSC Code:
12352200
Form:
lyophilized
Assay:
≥95% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in HEK 293 cells
Mol wt:
calculated mol wt 17.7 kDa, observed mol wt 25-32 kDa by SDS-PAGE (reducing) (His is the predicted N-terminal .)
biological source
human
recombinant
expressed in HEK 293 cells
tag
6-His tagged (N-terminus)
assay
≥95% (SDS-PAGE)
form
lyophilized
mol wt
calculated mol wt 17.7 kDa, observed mol wt 25-32 kDa by SDS-PAGE (reducing) (His is the predicted N-terminal .)
packaging
pkg of 10 μg
impurities
<1 EU/μg endotoxin (LAL test)
UniProt accession no.
shipped in
wet ice
storage temp.
−20°C
Gene Information
human ... FASLG(356)
General description
Fas ligand also known as FasL, CD178, CD95L, or TNFSF6, is a 40 kDa homotrimeric type-II transmembrane protein that belongs to the tumor necrosis factor (TNF) family. The FASL gene was mapped to human chromosome 1q24.3. It spans a length of 8 kb and contains 4 exons. It is found to be expressed on T and NK cells that are activated.
Biochem/physiol Actions
The binding of Fas ligand to its receptor induces apoptosis. Fas ligand/receptor interactions play an important role in the regulation of the immune system and the progression of cancer. Its functions include T-cell homeostasis, cytotoxic T-cell activity, immune privilege, maternal tolerance, tumor counterattack. Defective Fas-mediated apoptosis may lead to oncogenesis as well as drug resistance in existing tumors. Germline mutation of Fas is associated with autoimmune lympho proliferative syndrome (ALPS), a childhood disorder of apoptosis.
Physical form
Lyophilized from 0.22 μm filtered solution in 50 mM tris, 100 mM glycine, pH 7.0. Normally Mannitol or Trehalose is added as protectants before lyophilization.
Preparation Note
Centrifuge the vial prior to opening. Reconstitute in sterile PBS, pH 7.4 to a concentration of 50 μg/mL. Do not vortex. This solution can be stored at 2-8°C for up to 1 month. For extended storage, it is recommended to store at -20°C.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
法规信息
新产品
此项目有
Metalloproteinase-mediated release of human Fas ligand.
Kayagaki N, et al.
The Journal of Experimental Medicine, 182(6), 1777-1783 (1995)
Statistical colocalization of genetic risk variants for related autoimmune diseases in the context of common controls.
Fortune M D, et al.
Nature Genetics, 47(7), 839-839 (2015)
Dominant interfering Fas gene mutations impair apoptosis in a human autoimmune lymphoproliferative syndrome.
Fisher G H, et al.
Cell, 81(6), 935-946 (1995)
Insights into the molecular regulation of FasL (CD178) biology.
Lettau M, et al.
European Journal of Cell Biology, 90(6-7), 456-466 (2011)
T Takahashi et al.
International immunology, 6(10), 1567-1574 (1994-10-01)
Fas ligand (FasL) is a 40 kDa type II membrane protein belonging to the tumor necrosis factor family, which induces apoptosis by binding to its receptor, Fas. In this report, we isolated the chromosomal gene for human FasL. The human
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