T8690
硫氧还蛋白 人
≥90% (SDS-PAGE), recombinant, expressed in E. coli (N-terminal histidine tagged), essentially salt-free, lyophilized powder, ≥5 U/mg
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关于此项目
Form:
essentially salt-free, lyophilized powder
Assay:
≥90% (SDS-PAGE)
Biological source:
human
Recombinant:
expressed in E. coli (N-terminal histidine tagged)
Mol wt:
14 kDa
biological source
human
recombinant
expressed in E. coli (N-terminal histidine tagged)
assay
≥90% (SDS-PAGE)
form
essentially salt-free, lyophilized powder
specific activity
≥5 U/mg
mol wt
14 kDa
technique(s)
activity assay: suitable
solubility
water: soluble 0.50—0.60 mg/mL, clear to slightly hazy, colorless to faint yellow or tan
suitability
suitable for molecular biology
UniProt accession no.
application(s)
cell analysis
storage temp.
−20°C
Quality Level
Gene Information
human ... TXN(7295)
General description
从Jurkat细胞cDNA克隆得到。与ADF/Trx（GenBank®登记号X77584）和来自人胎盘的硫氧还蛋白（GenBank®登记号AF085844）相同。
硫氧还蛋白（TXN）是一种12kda的普遍存在蛋白。表达基因位于人染色体9q34.3上。TXN主要位于细胞质中。硫氧还蛋白在活性部位含有一个氧化还原活性二硫醇。它以几种形式存在，最常见的是细胞质（TRX1）和线粒体（TRX2）形式。
Application
人硫氧还蛋白可用于体外硫氧还蛋白测定。该产品还可用于评估硫氧还蛋白还原酶（TRXR）的酶活性。
人硫氧还蛋白已用于：
- 丝裂素活化蛋白激酶激酶4（MKK4）的体外激酶测定
- 以研究其在人细胞中转录调控、RNA处理和核孔功能中的作用
- 来自心脏特异性转基因(Tg)-Trx1小鼠心脏的Trx1还原性靶蛋白的蛋白质组学鉴定
Biochem/physiol Actions
人硫氧还蛋白是一种重组的N-末端组氨酸标记蛋白。该产品可在淋巴细胞、成纤维细胞以及多种人实体瘤细胞系中诱导增殖。该产品还能刺激正常和白血病B细胞的生长、改变AP-1的转录活性并抑制凋亡信号调节激酶1（ASK1）。硫氧还蛋白（TRX）在保护细胞免受亲电试剂（一种细胞毒性物质）的侵害中起作用。
介导蛋白中二硫键的还原。
硫氧还蛋白（TXN）具有二硫化物还原活性。它可作为细胞因子以减轻炎症。TXN可作为活性氧中间体（ROI）的清除剂而在各种氧化应激下产生。硫氧还蛋白能诱导细胞增殖以减少氧化应激。TXN是多种癌症的预后生物标志物，如肺癌、胰腺癌和肝细胞癌。它有助于蛋白质还原、组织发育和氧化还原调节。
Other Notes
在25℃的胰岛素还原测定中，一单位在1 min内可引起1.0的ΔA650。
Legal Information
GenBank is a registered trademark of United States Department of Health and Human Services
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
常规特殊物品
此项目有
Cexiong Fu et al.
Molecular & cellular proteomics : MCP, 8(7), 1674-1687 (2009-05-07)
Thioredoxin 1 (Trx1) is a key redox modulator that is functionally conserved across a wide range of species, including plants, bacteria, and mammals. Using a conserved CXXC motif, Trx1 catalyzes the reduction of cysteine disulfides and S-nitrosothiols. In contrast to
TXN (thioredoxin)
Chen Z, et al.
Atlas of Genetics and Cytogenetics in Oncology and Haematology (2010)
Walter H Watson et al.
Toxicological sciences : an official journal of the Society of Toxicology, 78(1), 3-14 (2003-12-24)
Thioredoxins (Trx) are members of an evolutionarily conserved family of redox-active proteins containing a conserved active site dithiol motif. Trx supports diverse reduction reactions, including several of direct toxicologic interest, but relatively little information is available concerning the roles of
Thioredoxin activates MKK4-NFkappaB pathway in a redox dependent manner to control manganese superoxide dismutase gene expression in endothelial cells
Kundumani-Sridharan V, et al.
The Journal of Biological Chemistry, jbc-M115 (2015)
Identification of novel nuclear targets of human thioredoxin 1
Wu C, et al.
Molecular and Cellular Proteomics, mcp-M114 (2014)
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