T9698
硫氧还蛋白还原酶 来源于大鼠肝脏
buffered aqueous glycerol solution, ≥100 units/mg protein (Bradford)
别名:
NADPH：氧化型硫氧还蛋白氧化还原酶, 硫氧还蛋白：NADP + 氧化还原酶
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关于此项目
化学文摘社编号:
UNSPSC Code:
12352200
NACRES:
NA.32
MDL number:
Form:
buffered aqueous glycerol solution
Assay:
≥90% (GE)
Biological source:
rat liver
Mol wt:
55—67 kDa
产品名称
硫氧还蛋白还原酶 来源于大鼠肝脏, buffered aqueous glycerol solution, ≥100 units/mg protein (Bradford)
biological source
rat liver
assay
≥90% (GE)
form
buffered aqueous glycerol solution
specific activity
≥100 units/mg protein (Bradford)
mol wt
55—67 kDa
technique(s)
activity assay: suitable
impurities
Glutathione reductase
solubility
water: soluble
suitability
suitable for molecular biology
UniProt accession no.
shipped in
dry ice
storage temp.
−20°C
Quality Level
Gene Information
rat ... Txnrd1(58819)
Application
大鼠肝脏硫氧还蛋白还原酶可利用人红细胞的还原能力来研究 α-硫辛酸的摄取和还原。该产品还可用于利用硫氧还蛋白研究细胞外基质中转谷氨酰胺酶 2 (TG2) 的激活机制。
Biochem/physiol Actions
哺乳动物来源的硫氧还蛋白还原酶含有硒代半胱氨酸残基，对酶的活性是必需的。它与过氧化氢酶、谷胱甘肽过氧化物酶和超氧化物歧化酶一起存在于哺乳动物细胞中，是一种抗氧化酶，有助于清除细胞中的活性氧 (ROS)。例如，过量的一氧化氮 (NO) 与谷胱甘肽形成复合物，形成 S-亚硝基谷胱甘肽加合物 (GS-NO)。这可以被硫氧还蛋白还原酶直接裂解。细胞中的另一种有害氧化剂过氧化氢也可被哺乳动物 TrxR 直接还原。
硫氧还蛋白还原酶 (TrxR) 是一种 NADPH 依赖性氧化还原酶，每个亚基含有一个 FAD，可还原氧化硫氧还蛋白 (Trx) 中的活性站点二硫键。
硫氧还蛋白还原酶 (TrxR) 是一种 NADPH 依赖性氧化还原酶，每个亚基含有一个 FAD，可还原氧化硫氧还蛋白 (Trx) 中的活性站点二硫键。与原核生物、植物或酵母中的 35 kDa 相比，哺乳动物来源同工酶的分子量在 55-67 kDa 之间变化。哺乳动物酶的底物特异性比原核生物酶广泛得多，同时还原哺乳动物和 大肠杆菌 硫氧还蛋白以及非二硫键底物，如亚硒酸盐、硫辛酸、脂质氢过氧化物和过氧化氢。
硫氧还蛋白还原酶是一种普遍存在的酶，可通过NADPH催化硫氧还蛋白活性位点二硫化。该产品还减少和再生泛素，一种强大的抗氧化剂。
General description
研究领域：细胞信号传导
Other Notes
在 pH 7.0，25 °C 下，一个单位每分钟将在 412nm 处吸光度增加1.0（当在仅含 DTNB [Sigma No.D8130] 作为底物的非偶联测定中测量时）。
Physical form
溶于 50 mM Tris-HCl (pH 7.5)、300 mM NaCl、1 mM EDTA 和 10% 甘油中。
存储类别
12 - Non Combustible Liquids
wgk
WGK 1
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, multi-purpose combination respirator cartridge (US)
法规信息
低风险生物材料
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Cellular oxidative stress is countered by enzymatic scavengers and antioxidant modulators against reactive oxygen species damage.
氧化应激，在一部分上，是由多种细胞过程产生的活性氧介导，并由诸如酶清除剂或抗氧化剂调节剂等细胞抗氧化剂机制所控制的。自由基，如活性氧，会通过细胞引起细胞损伤。
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