描述
wavelength 595 nm
total test time 2 min
working reagent stability 18-26°C for 2 weeks
dynamic range 50 mg/dL 0.5 g/L
质量水平
参数
18-26 °C temperature
50 μL sample volume
储存温度
2-8°C
一般描述
蛋白质测定是生化研究最常见的实验操作之一。微量总蛋白试剂盒提供了一种简单、快速、高灵敏度的方法，只需 50μ L 或更少的样品。
应用
可测定多达0.5mg/ml 的蛋白样品浓度。
微量总蛋白试剂盒已被用于测定:
- 通过Bradford 法测定细菌匀浆液中的总蛋白
- 通过Bradford 微量检测法测定组织提取物中的总蛋白含量
- 血清总蛋白
- 通过Bradford 法测定叶片中的可溶性蛋白质
比色法，终点法（Endpoint）
生化/生理作用
亮蓝G-250(考马斯亮蓝)在酸性条件下，与蛋白质反应，形成蓝色蛋白质染料复合物。该复合物在595nm处的吸光度与蛋白质含量成正比。
微量总蛋白试剂盒可用于测定溶液中蛋白质的浓度。
警示用语：
Danger
危险分类
Acute Tox. 4 Dermal - Acute Tox. 4 Inhalation - Acute Tox. 4 Oral - Aquatic Chronic 2 - Eye Dam. 1 - Flam. Liq. 3 - Met. Corr. 1 - Skin Corr. 1B - Skin Sens. 1 - STOT SE 1
靶器官
Eyes,Central nervous system
储存分类代码
3 - Flammable liquids
闪点(°F)
132.8 °F
闪点(°C)
56 °C
法规信息
高风险级别生物产品--人源产品
危险化学品
历史批次信息供参考:
分析证书(COA)
Lot/Batch Number
Jason G Lieberthal et al.
The Journal of rheumatology, 40(5), 674-683 (2013-04-03)
Glomerulonephritis (GN) is common in antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV), but tools for early detection of renal involvement are imperfect. We investigated 4 urinary proteins as markers of active renal AAV: alpha-1 acid glycoprotein (AGP), kidney injury molecule-1 (KIM-1)
Digital camera-based lipase biosensor for the determination of paraoxon
Pohanka M, et al.
Sensors and Actuators B, Chemical, 273, 610-615 (2018)
Effect of a dietary mixture of beneficial bacteria on growth performance, health condition, chemical composition, and water quality of Nile Tilapia, Oreochromis niloticus fingerlings
Khalafalla MM, et al.
Journal of Aquatic Food Product Technology, 29(8), 823-835 (2020)
Nagham R El Saidy et al.
Environmental monitoring and assessment, 192(12), 779-779 (2020-11-25)
The remediation of wastewater (WW) is a promising solution for limited water sources. This study aimed to evaluate rice straw (RS) and zeolite (Z) as bioadsorbents for the removal of pollutants, including heavy metals (HMs) (cadmium [Cd], nickel [Ni], and
Differences in antioxidant mechanisms in grapevines subjected to drought and enhanced UV-B radiation.
Doupis G, et al.
Emirates Journal of Food and Agriculture, 24(6) (2012)
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