V3513
Vascular Endothelial Growth Factor 120 from mouse
>97% (SDS-PAGE), recombinant, expressed in E. coli, lyophilized powder
别名:
VEGF120, Vasculotropin
biological source
mouse
recombinant
expressed in E. coli
assay
>97% (SDS-PAGE)
form
lyophilized powder
potency
~2-4 ng/mL EC50
mol wt
predicted mol wt 14 kDa
storage condition
avoid repeated freeze/thaw cycles
impurities
endotoxin, tested
UniProt accession no.
storage temp.
−20°C
Gene Information
mouse ... Vegfa(22339)
Application
Vascular Endothelial Growth Factor 120 from mouse can be used as a test reagent for treating cells grown in a flat-bottom 24-well assay plate, which in turn is utilized for studying whether the Lipopolysaccharide can induce apoptosis in porcine aortic endothelial cells and stimulates heat shock protein (Hsp)70, Hsp32 as well as vascular endothelial growth factor (VEGF) production or not. The product was used to study the culture conditions that differentiate mouse embryonic stem (ES) cells into endothelial cells.
Biochem/physiol Actions
Mitogen for endothelial cells, enhances vascular permeability, activates and chemoattracts monocytes, and is a procoagulant.
Vascular Endothelial Growth Factor 120 also referred to as VEGF 120 or Vasculotropin is a basic protein that induces endothelial cell growth, vasculogenesis and angiogenesis. VEGF also enhances the growth of endothelial cells isolated from cerebral cortex, fetal and adult aorta as well as human umbilical vein. It serves as a significant mediator in the pathogenesis of endothelial dysfunction in diabetes.
Physical form
Lyophilized from a 0.2 μm filtered solution in 30% acetonitrile and 0.1% TFA containing 0.25 mg bovine serum albumin.
Analysis Note
The biological activity is measured by its ability to stimulate 3H-thymidine incorporation in human umbilical vein endothelial cells.
Other Notes
120 amino acid residue variant of mouse VEGF.
存储类别
11 - Combustible Solids
wgk
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
ppe
Eyeshields, Gloves, type N95 (US)
法规信息
新产品
此项目有
Chiara Bernardini et al.
Cell stress & chaperones, 10(4), 340-348 (2005-12-13)
Lipopolysaccharide (LPS) is a highly proactive molecule that causes in vivo a systemic inflammatory response syndrome and activates in vitro the inflammatory pathway in different cellular types, including endothelial cells (EC). Because the proinflammatory status could lead to EC injury
D R Cha et al.
Kidney international. Supplement, 77, S104-S112 (2000-09-21)
Vascular endothelial growth factor (VEGF) is a potent cytokine that is considered to be an important mediator in the pathogenesis of endothelial dysfunction in diabetes. This study investigates the effect of high glucose on the signaling and production of VEGF
Molecular and biological properties of the vascular endothelial growth factor family of proteins.
N Ferrara et al.
Endocrine reviews, 13(1), 18-32 (1992-02-01)
C J Robinson et al.
Journal of cell science, 114(Pt 5), 853-865 (2001-02-22)
Vascular endothelial growth factor (VEGF) is a secreted mitogen highly specific for cultured endothelial cells. In vivo VEGF induces microvascular permeability and plays a central role in both angiogenesis and vasculogenesis. VEGF is a promising target for therapeutic intervention in
G Neufeld et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 13(1), 9-22 (1999-01-05)
Vascular endothelial growth factor (VEGF) is a highly specific mitogen for vascular endothelial cells. Five VEGF isoforms are generated as a result of alternative splicing from a single VEGF gene. These isoforms differ in their molecular mass and in biological
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