BSTFA + TMCS，99:1

BSTFA is a stronger silylating reagent as compared to BSA. In most cases, BSTFA is used in place of BSA as the reaction products are more volatile avoiding interference with early peaks in a chromatogram. BSTFA along with TMCS is considered to be a suitable reagent for quantitative reactions.

It was used in the derivatization of water during determination of trace levels of bisphenol A from plastic water containers, using solid-phase microextraction (SPME) coupled with GC-MS.

Suitable for the derivitazation of alkylphosphonic acids and aminoalkylphosphonic acids, aminothiols, bilirubins, carboxylic acids sodium salts, glycerophospholipids, homovanillic acids and vanillylmandelic acid , 3-O-methyldopamine and 4-O-methyldopamine, phosphoryl and phosphono ethanolamine, polyphosphoinositides, steroid phosphates, tryptamines, tyrosine, tryptophan and metabolites.

Reagent for isothiocyanate (amino compounds), oxime, trimethylsilyl (TMS), trimethylsilyl deacylated polyphosphoinositides and trimethylsilyl glycerophosphate esters.