一般描述
Mono 色谱柱是高效、pH 稳定的色谱柱，设计用于蛋白质、多肽和多核苷酸的高效离子交换分离，应用范围包括肽图谱和单克隆抗体纯化。这些色谱柱的独特属性是基于单柱支撑-一种珠状亲水性材料，具有任何色谱支撑体中最窄的粒度分布。这种单分散性允许在相对较低的反向压力下获得较高的流速。
应用
Mono 色谱柱用于包括肽图谱和单克隆抗体纯化在内的应用。Mono Q 柱已被用于开发一种高分辨率分析和纯化寡核苷酸二硫代产物的方法，并研究 DNA 限制性片段的分离。
法律信息
Mono Q is a registered trademark of Cytiva
Xianbin Yang et al.
Analytical biochemistry, 306(1), 92-99 (2002-06-19)
A method using a strong anion-exchange liquid-chromatography column, Mono-Q, has been developed for high-resolution analysis and purification of oligonucleotide dithioates, which were synthesized by an automated, solid-phase, phosphorothioamidite chemistry. High-resolution separation of oligonucleotide phosphorodithioates from monothiophosphate impurities was obtained. High-resolution
E Westman et al.
Analytical biochemistry, 166(1), 158-171 (1987-10-01)
Separation of DNA restriction fragments by FPLC ion-exchange chromatography on Mono Q and Mono P columns was investigated. The columns were found to be particularly suitable for the separation of fragments up to 500-600 bp long. Larger fragments can also
Chromatograms
application for HPLC
application for HPLC
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