567513-U
Discovery® HS F5 (5 µm) HPLC Columns
L × I.D. 5 cm × 4.6 mm, HPLC Column
别名:
Discovery Pentafluorophenyl PFP HPLC Column
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关于此项目
UNSPSC Code:
41115700
eCl@ss:
32110501
NACRES:
SB.52
L × i.d.:
5 cm × 4.6 mm
Particle size:
5 μm
Matrix active group:
PFP (pentafluorophenyl) phase
Pore size:
120 Å
Matrix:
silica gel, high purity, spherical particle platform, fully porous particle
产品名称
Discovery ® HS F5 HPLC 色谱柱, 5 μm particle size, L × I.D. 5 cm × 4.6 mm
material
stainless steel column
agency
suitable for USP L43
product line
Discovery®
feature
endcapped
manufacturer/tradename
Discovery®
packaging
1 ea of
extent of labeling
12% Carbon loading
parameter
≤70 °C temp. range, 400 bar pressure (5801 psi)
technique(s)
HPLC: suitable, LC/MS: suitable
L × I.D.
5 cm × 4.6 mm
surface area
300 m2/g
surface coverage
4 μmol/m2
impurities
<10 ppm metals
matrix
silica gel, high purity, spherical particle platform, fully porous particle
matrix active group
PFP (pentafluorophenyl) phase
particle size
5 μm
pore size
120 Å
operating pH range
2-8
application(s)
food and beverages
separation technique
reversed phase
Quality Level
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General description
将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。
Application
- Optimization and validation of a bioanalytical HPLC-UV technique for simultaneous determination of underivatized phenylalanine and tyrosine in the blood for phenylketonuria diagnosis and monitoring.: This study utilizes the Discovery® HS F5 HPLC Column to develop an HPLC-UV method for the simultaneous determination of phenylalanine and tyrosine in blood, significantly advancing the diagnostic and monitoring capabilities for phenylketonuria (Rabti et al., 2024).
- A new RP-HPLC method as an auxiliary tool for optimization of sample preparation procedures for tracing of PPCPs of different hydrophilicities.: Demonstrates the application of the Discovery® HS F5 HPLC Column in optimizing sample preparation techniques for the detection of pharmaceuticals and personal care products in environmental samples, enhancing the analysis of diverse hydrophilic compounds (Portillo-Castillo et al., 2021).
- "Heart-cut" bidimensional achiral-chiral liquid chromatography applied to the evaluation of stereoselective metabolism, in vivo biological activity and brain response to chiral drug candidates targeting the central nervous system.: Utilizes the Discovery® HS F5 HPLC Column in a novel bidimensional chromatographic setup to study the stereoselective metabolism of CNS-targeting chiral drugs, contributing to a deeper understanding of their pharmacological profiles (Battisti et al., 2016).
- HPLC method development, validation, and impurity characterization of a potent antitumor indenoisoquinoline, LMP776 (NSC 725776).: This research employs the Discovery® HS F5 HPLC Column for developing an HPLC method to identify and characterize impurities in the antitumor drug LMP776, ensuring drug purity and compliance with regulatory standards (Wang et al., 2016).
- Development studies on determination of preservatives decomposition products.: Showcases the application of the Discovery® HS F5 HPLC Column in the analysis of decomposition products of preservatives, enhancing the safety assessments of food and pharmaceutical products (Zabrzewska et al., 2014).
Features and Benefits
- 独特的选择性
- 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
- 峰形极佳
- 稳定性好、低流失，适用于 LC-MS 分析
- 粒径范围从 3μm 到 10μm 可供选择
Legal Information
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Jian Mao et al.
Se pu = Chinese journal of chromatography, 27(2), 216-219 (2009-07-25)
A simple method was developed for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline (6-OH-MTHbetaC), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) in rat brain by high performance liquid chromatography with electrochemical detection (HPLC-ECD). The separation of the sample was performed on a Discovery
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