产品名称
Discovery ® HS F5 HPLC 色谱柱, 5 μm particle size, L × I.D. 15 cm × 4.6 mm
物料
stainless steel column
质量水平
Agency
suitable for USP L43
产品线
Discovery®
特点
endcapped
制造商/商品名称
Discovery®
包装
1 ea of
标记范围
12% Carbon loading
参数
≤70 °C temp. range
400 bar pressure (5801 psi)
技术
HPLC: suitable
LC/MS: suitable
长度 × 内径
15 cm × 4.6 mm
表面积
300 m2/g
表面覆盖度
4 μmol/m2
杂质
<10 ppm metals
基质
silica gel, high purity, spherical particle platform
fully porous particle
基质活性基团
PFP (pentafluorophenyl) phase
粒径
5 μm
孔径
120 Å
工作pH范围
2-8
应用
food and beverages
分离技术
reversed phase
正在寻找类似产品? 访问 产品对比指南
一般描述
将 C18 柱方法转换至 Discovery® HS F5 柱的指导原则：
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
通常，碱性化合物在 HS F5 色谱柱上的保留时间比在 C18 柱上更长。在 C18 柱上流动相中的有机相含量提高 5% 至 10% 通常可获得与 HS F5 柱类似的保留值。使用其他化合物所得的结果差异则很大。然而 log Po/w 值小于 2.5 的化合物在 HS F5 柱上的保留时间通常要长于 C18 柱。保留时间的差异程度与化合物的 logP o/w 值密切相关。
Discovery® HS F5 键合相可提供与 C18 色谱柱截然不同的反相分离。由于 F5 色谱柱上各种化合物通常会在与 C18 相同的时间范围内洗脱出来，使得大部分的 C18 方法可轻松转换到 F5 上。
应用
- Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.：利用Discovery® HS F5 HPLC色谱柱出色分离大米中各种砷形态，改进食品安全性分析，保证健康监管合规性(Baba et al., 2014)。
- High-performance liquid chromatographic determination of tiapride and its phase I metabolite in blood plasma using tandem UV photodiode-array and fluorescence detection.：使用Discovery® HS F5 HPLC色谱柱进行硫必利及其代谢物的同步灵敏检测，提供药物监测和药物动力学研究参考价值。体现本品的多用途(Nobilis et al., 2011)。
- Simultaneous quantification of nicotine and metabolites in rat brain by liquid chromatography-tandem mass spectrometry.：利用Discovery® HS F5 HPLC色谱柱准确检测大鼠脑中尼古丁及其代谢产物，为药物成瘾和神经药理学研究提供了重要数据(Vieira-Brock et al., 2011)。
- Biomarker discovery in biological specimens (plasma, hair, liver and kidney) of diabetic mice based upon metabolite profiling using ultra-performance liquid chromatography with electrospray ionization time-of-flight mass spectrometry.：用Discovery® HS F5 HPLC色谱柱找出各种糖尿病小鼠生物样本中的生物标志物，贡献分子水平的糖尿病知识(Tsutsui et al., 2011)。
- Practical analytical approach for the identification of biomarker candidates in prediabetic state based upon metabonomic study by ultraperformance liquid chromatography coupled to electrospray ionization time-of-flight mass spectrometry.：在代谢组学方法中采用Discovery® HS F5 HPLC色谱柱以鉴定糖尿病早期生物标志物，促进预防医学和代谢研究(Tsutsui et al., 2010)。
特点和优势
- 独特的选择性
- 保留能力与 C18 柱类似（有时需要洗脱能力更强的流动相）
- 峰形极佳
- 稳定性好、低流失，适用于 LC-MS 分析
- 粒径范围从 3μm 到 10μm 可供选择
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
Chromatographic separation of arsenic species with pentafluorophenyl column and application to rice.
Koji Baba et al.
Journal of chromatography. A, 1354, 109-116 (2014-06-21)
Arsenic species, including arsenous acid, arsenic acid, methylarsonic acid, and dimethylarsinic acid, were determined using HPLC-ICPMS. The species were separated with a Discovery HS F5 column and a simple, volatile, and isocratic mobile phase of 0.1% (v/v) formic acid and
Federica Pellati et al.
Journal of pharmaceutical and biomedical analysis, 48(2), 254-263 (2007-12-14)
In this study a pentafluorophenylpropyl (PFPP) stationary phase was applied to the fast and reliable qualitative and quantitative analysis of ephedrine alkaloids in Ephedra plant material and derivatives. A Discovery HS F5 column (150mmx4.6mm i.d., 5microm) was used, with an
Ilia Brondz et al.
Journal of pharmaceutical and biomedical analysis, 43(3), 937-944 (2006-11-03)
The drug primaquine diphosphate is used for causative treatment of malaria. Using HPLC-MS and GC-MS, this research group was previously able to show that the main contaminant of primaquine is the positional isomer quinocide [I. Brondz, D. Mantzilas, U. Klein
Federica Pellati et al.
Journal of chromatography. A, 1165(1-2), 58-66 (2007-08-07)
In this study, the chromatographic performance of a pentafluorophenylpropyl (PFPP) stationary phase was evaluated for the rapid separation of phenethylamine alkaloids (i.e. (+/-)-octopamine, (+/-)-synephrine, tyramine, N-methyltyramine and hordenine) in Citrus aurantium plant material (fruits and peel), various Citrus species, extracts
Jian Mao et al.
Se pu = Chinese journal of chromatography, 27(2), 216-219 (2009-07-25)
A simple method was developed for the analysis of 6-hydroxy-1-methyl-1,2,3,4-tetrahydro-beta-carboline (6-OH-MTHbetaC), 5-hydroxytryptamine (5-HT) and 5-hydroxyindole acetic acid (5-HIAA) in rat brain by high performance liquid chromatography with electrochemical detection (HPLC-ECD). The separation of the sample was performed on a Discovery
Chromatograms
application for HPLC
application for SPE, application for HPLC
application for SPE, application for HPLC
application for HPLC
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持