产品名称
TSKgel® Chelate-5PW HPLC Column, phase iminodiacetic acid, L × I.D. 7.5 cm × 7.5 mm, 10 μm particle size
物料
stainless steel
产品线
TSKgel®
制造商/商品名称
Tosoh Chelate-5PW, 08645
标记范围
20 μmol/mL resin ligand density
参数
≤20% organic solvent
≤3 M salt concentration
≤45 °C temperature
1.2 mL/min flow rate
10 bar max. pressure
技术
HPLC: suitable
长度 × 内径
7.5 cm × 7.5 mm
基质
polymer particle platform
基质活性基团
iminodiacetic acid phase
粒径
10 μm
孔径
1000 Å
工作pH值
2-12
相容性
technique used for affinity HPLC
分离技术
affinity
一般描述
High efficiency, resin-based TSKgel affinity columns (10 μm particles) separate or purify many enzymes and other proteins.
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
TSKgel Chelate-5PW columns utilize the tendency of iminodiacetic acid (IDA) to form a complex with metal ions, such as Zn2+, Ni2+ and Cu2+. The column is loaded with divalent metal ions by chelation. Peptides and proteins containing histidine residues will normally adsorb to these chelated ions at neutral pH. The retained compounds can be eluted with buffer containing imidazole or glycine. The key to making successful use of this retention mechanism is the proper selection of metal ions for chelation and the elution buffer to desorb the analytes. In general, Cu2+, interacts better with proteins; however, resolution is usually enhanced with Zn2+. A gradient mobile phase containing increasing imidazole or glycine concentrations is used to elute the retained compounds. Alternatively, a decreasing pH gradient can be used for elution purposes as well.
Applications include: immunoglobulins, transferrin, lectins, milk proteins, membrane proteins and peptides.
法律信息
TSKgel is a registered trademark of Tosoh Corporation
法规信息
新产品
此项目有
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持