技术
HPLC: suitable
试验参数
column: Ascentis C18, 10 cm x 2.1 mm I.D., 5 μm particles (581326-U)
mobile phase: [A] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:water; [B] 50:50 (v/v) 50 mM formic acid, pH 3.0 with acetic acid:acetonitrile
gradient: 20 to 50% B in 15 min
flow rate: 0.2 mL/min
column temp.: ambient
detector: ESI (+)
injection: 4 μL
sample: as indicated in 10 mM formic acid
适用性
application for HPLC
应用
life science and biopharma
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
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