HPLC Analysis of Tetracycline Antibiotics on Ascentis^® Phenyl

技术

HPLC: suitable

试验参数

column: Ascentis Phenyl, 15 cm x 4.6 mm I.D., 5 μm particles (581616-U)
mobile phase: [A] 0.1% ammonium acetate; [B] acetonitrile with 0.1% ammonium acetate; (80:20, A:B)
flow rate: 1.0 mL/min
column temp.: 35 °C
detector: UV, 353 nm
injection: 10 μL
sample: 50 μg/mL each in 80:20, water:methanol

适用性

application for HPLC

应用

pharmaceutical (small molecule)