技术
HPLC: suitable
试验参数
column: Ascentis Si, 15 cm x 4.6 mm I.D., 5 μm particles (581512-U)
mobile phase: [A] hexane; [B] ethyl acetate
gradient: 10 to 30% B in 10 min; held for 2 min; to 10% B in 1 min
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 290 nm
injection: 10 μL
sample: as indicated in 96:4 (hexane:isopropanol)
适用性
application for HPLC
应用
vitamins, nutraceuticals, and natural products
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
(±)-α-Tocopherol, analytical standard 100 mg/mL
Menadione (K3), analytical standard
Cholecalciferol (D3), analytical standard
(+)-γ-Tocopherol, analytical standard 200 mg/mL
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