技术
HPLC: suitable
试验参数
column: Ascentis RP-Amide, 15 cm x 4.6 mm I.D., 5 μm particles (565324-U)
mobile phase: [A] 0.1% ammonium acetate in water, pH 4.5 with acetic acid; [B] acetonitrile
gradient: 2 to 8% B in 3 min; to 20% B in 9 min; held for 2 min; to 2% B in 0.1 min; held for 0.9 min
flow rate: 1.0 mL/min
column temp.: 30 °C
detector: UV, 275 nm
injection: 10 μL
sample: as indicated in 0.1% ammonium acetate in water (pH 4.5 with acetic acid)
适用性
application for HPLC
应用
clinical
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Uric acid 50 mg/mL
Xanthine
7-Methylxanthine
1-Methyluric acid
1-Methylxanthine 20 mg/mL
Theobromine 30 mg/mL
1,7-Dimethyluric acid
1,7-Dimethylxanthine 50 mg/mL
Theophylline
β-(Hydroxyethyl)theophylline 50 mg/mL
Caffeine
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