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Merck
CN

G005593

HPLC Analysis of Flavonoids in Lime Juice on Ascentis® Express C18

application for HPLC, application for SPE

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技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: lime juice hand-squeezed in laboratory, centrifuged and filtered through a 0.45 μm nylon filter disc (Acrodisc)
column: Ascentis Express C18, 5 cm x 4.6 mm I.D., 2.7 μm particles (53826-U)
mobile phase: [A] water/formic acid (99.9:0.1); [B] water/acetonitrile/isopropanol/formic acid (39.9:20:40:0.1)
gradient: 10% B for 3 min; 10 to 34% B in 30 min; 34 to 100% B in 1 min; held at 100% B for 6 min; 100 to10% B in 1 min
flow rate: 0.8 mL/min
column temp.: ambient
detector: PDA: 190–370 nm; chromatogram extracted at 283 nm
detector: ESI-MS: mass spectral range, 250-700 m/z; interval, 0.5 s; scan speed, 938 amu/s; nebulizing gas (N2) flow, 1.5 L/min; (interface temperature, 350 °C; heat block, 300 °C; desolvation line (DL) temperature, 300 °C; DL voltage, 34 V; probe voltage, +4.5 kV; Qarray voltage, 1.0 V; RF voltage, 90 V; detection gain, 1.05 kV)
injection: 2 μL

适用性

application for HPLC
application for SPE

应用

food and beverages

分析说明

Flavonoids are polyphenolic compounds ubiquitous in nature and classified, according to their chemical structure, into flavonols, flavones, flavanones, isoflavones, catechins, anthocyanidins and chalcones. They are widely present in fruits, vegetables, leaves, nuts, seeds, bark and beverages (tea, coffee, beer, wine and fruit drinks).

法律信息

Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

说明
    Eriocitrin
    Narirutin
    Hesperidin

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

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