技术
HPLC: suitable
试验参数
column: Discovery C18, 25 cm x 4.6 mm I.D., 5 μm particles (504971)
mobile phase: [A] 1 mM potassium phosphate, monobasic; [B] acetonitrile
gradient: 5 to 45% B in 18 min; 45 to 80% B in 7 min; held at 80% B for 3 min
flow rate: 1.5 mL/min
pressure: 3191 psi (220 bar)
column temp.: 27 °C
detector: UV, 227 nm
injection: 20 μL
sample: 20 μg/mL in water: acetonitrile (86:14)
适用性
application for HPLC
应用
vitamins, nutraceuticals, and natural products
分析说明
This application demonstrates the suitability of Discovery C18 for the efficient separation of withania extract by the USP monograph.
法律信息
Discovery is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Withanoside IV
Withanoside V
Withaferin A
12-Deoxywithastramonolide
Withanolide A
Withanolide B
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持