技术
HPLC: suitable
试验参数
column: Ascentis Express HILIC, 10 cm x 3.0 mm I.D., 2.7 μm particles (53970-U)
mobile phase: [A] acetonitrile; [B] water with 0.1% formic acid, pH 3.5 with ammonium formate; (75:25, A:B)
flow rate: 0.4 mL/min
pressure: 1350 psi 93 bar
temp.: 25 °C
detector: MS, ESI (+), TIC, m/z 150-300
injection: 5 μL
sample: 10 μg/mL in 90:10, methanol:water
适用性
application for HPLC
应用
life science and biopharma
分析说明
L-methionine sulfoximine and L-buthionine sulfoximine are used in are used to prevent additional enzyme activity in Chinese hampster ovary (CHO) cell lines with supplemental Glutamine Synthase (GS). It is of interest to be able to monitor both purity of such molecules in order to control addition to cell media as well as for the assay of parent molecules during use. The analytes are highly polar and thus should be amenable to HILIC-LC-MS analysis. This first installment investigates the ability of Ascentis Express HILIC and Ascentis Express OH5 stationary phases for the separation of the methionine analog and related compounds as well as methionine and buthionine separations.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
L-Methionine sulfone
L-Methionine sulfoximine, suitable for cell culture
L-Methionine sulfoxide
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