技术
HPLC: suitable
试验参数
column: Ascentis Express RP-Amide, 10 cm x 4.6 mm I.D., 2.7 μm particles (53929-U)
mobile phase: [A] 0.2% formic acid; [B] methanol
gradient: 5% B for 2 min; to 90% B over 18 min; held at 90% B for 10 min
flow rate: 0.6 mL/min
column temp.: 35 °C
detector: UV, 254 nm
detector: MS (ion trap), m/z 100-1500
injection: 2 μL
sample: 1 mg/mL in methanol
适用性
application for HPLC
应用
life science and biopharma
pharmaceutical (small molecule)
分析说明
Optimization of a published method showing much better retention, peak shape and resolution on Supelco Ascentis Express RP-Amide 2.7 μm compared to a C18 phase. Isomers and small amounts of homologues are resolved and detectable with higher sensitivity.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Oenothein B, analytical standard
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持