技术
HPLC: suitable
试验参数
column: Ascentis Express RPA 15 cm x 4.6 mm, 5 μm particles (50773-U)
mobile phase: [A] 20 mM potassium phosphate, pH 7; [B] acetonitrile; (70:30, A:B)
flow rate: 4.0 mL/min
pressure: 4467 psi (308 bar)
column temp.: 26 °C
detector: UV, 254 nm
injection: 5 μL
sample: 1. Uracil (0.016 mg/mL), 2. Benzamide (0.161 mg/mL), 3. Aniline (0.161 mg/mL), 4. Cinnamyl Alcohol (0.016 mg/mL), 5. Dimethyl Phthalate (0.323 mg/mL), (6. 2-Nitroaniline (0.081 mg/mL), 7. 4′-Bromoacetanilide (0.040 mg/mL), 8. 2,2′-Biphenol (0.161 mg/mL), 9. 4,4′-Biphenol (0.040 mg/mL), in 50/50 Water/Acetonitrile)
适用性
application for HPLC
应用
general analytical
分析说明
This application shows a test mixture of compounds of widely varying polarity on the 5μm RPA Ascentis Express column.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Uracil, ≥99.0%
Benzamide, 99%
Aniline, ACS reagent, ≥99.5%
Cinnamyl alcohol, 98%
Dimethyl phthalate, ≥99%
2-Nitroaniline, 98%
4-Bromoacetanilide, 98%
2,2′-Biphenol, 99%
4,4′-Dihydroxybiphenyl, 97%
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