技术
UHPLC: suitable
试验参数
column: Ascentis Express C18, 10 cm x 2.1 mm I.D., 2.0 μm particles (50813-U)
mobile phase: [A] 10 mM ammonium formate, pH 3.0 with formic acid; [B] acetonitrile; (90:10, A:B)
flow rate: 0.7 mL/min
pressure: 1153 psi (795 bar)
column temp.: 35 °C
detector: UV, 254 nm
injection: 1 μL
sample: 50 μg/mL in 85:15, water:methanol
适用性
application for UHPLC
应用
pharmaceutical (small molecule)
分析说明
Methotrexate (MTX) has been selected as a tracer of surface contamination exposure of hospital staff to antineoplastic drugs (ANDs). Its analysis is an emerging facet of occupational medicine in hospital premises. Methotrexate and the related compounds aminopterin and folic acid are shown here separated by UHPLC on an Ascentis Express C18 column in less than two minutes. The rapid, high resolution separation means the method can be employed in hospitals or clinical settings to routinely monitor the contamination levels of surfaces.
法律信息
Ascentis is a registered trademark of Merck KGaA, Darmstadt, Germany
分析物
说明
Aminopterin, powder
Folic acid, analytical standard
Methotrexate hydrate, ≥98% (HPLC), powder
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