跳转至内容
Merck
CN

G006369

HPLC Analysis of PAHs in Canola Oil on SUPELCOSIL™ LC-PAH after SPE using Supelclean™ EZ-POP NP

application for SPE, application for HPLC

登录查看公司和协议定价

主要文件

查看所有文档
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助
技术服务
需要帮助？我们经验丰富的科学家团队随时乐意为您服务。
让我们为您提供帮助

技术

HPLC: suitable
solid phase extraction (SPE): suitable

试验参数

sample preparation: SPE (Solid Phase Extraction)
sample/matrix: Canola oil spiked with PAHs at 10 ng/g
SPE tube/cartridge: Supelclean™ EZ-POP NP, 12 mL, pk of 20 (54341-U)
condition: 10 mL acetone, dry under 10-15" mercury for 10 minutes
sample addition: 0.5 mL weighed accurately onto cartridge
elution: 15 mL acetonitrile
eluate post-treatment: concentrate to final volume of 0.5mL at 40 °C under nitrogen
column: SUPELCOSIL™ LC-PAH 25 cm x 4.6 mm I.D., 5 μm particles (58229)
mobile phase: [A] water [B] acetonitrile
gradient: 40% B held for 5 min; to 100% B in 15 min; held at 100% B for 12 min
flow rate: 2.4 mL/min
pressure: 2790 psi at start (192 bar)
column temp.: 25 °C
detector: Fluorescence, programmed.

适用性

application for HPLC
application for SPE

应用

food and beverages

分析说明

PAHs are ubiquitous in the environment, resulting from both natural and manmade sources of combustion. They can contaminate food crops through environmental exposure to particulates, automobile exhaust, etc. In the case of edible oils, additional PAHs can be introduced during actual production processes such as seed drying [1]. In this application, EZ-POP NP SPE is used to extract PAHs from soybean oil. The 16 PAHs designated in many US EPA methods (less acenaphthylene) were analyzed by HPLC-FLD after extraction from a spiked sample of oil.

其他说明

Detector: Naphthalene, Acenaphthene, Fluorene - 225nm/320nm; Phenanthrene, Anthracene - 250nm/368nm; Fluoranthene, Pyrene - 237nm/440nm; Benzo[a]anthracene, Chrysene - 265nm/380nm; Benzo[b]fluoranthene, Benzo[k]fluoranthene, Benzo[a]pyrene, Dibenzo[a,h]anthracene - 280nm/420nm; Benzo[g,h,i]perylene, Indeno[1,2,3-cd]pyrene, Benzo[b]chrysene - 300nm/466nm

法律信息

SUPELCOSIL is a trademark of Sigma-Aldrich Co. LLC
Supelclean is a trademark of Sigma-Aldrich Co. LLC

分析物

说明
    Naphthalene
    Acenaphthene
    Fluorene
    Phenanthrene
    Anthracene
    Fluoranthene
    Pyrene
    Benzo[a]anthracene
    Chrysene
    Benzo[b]fluoranthene
    Benzo[k]fluoranthene
    Benzo[a]pyrene
    Dibenzo[a,h]anthracene
    Benzo[g,h,i]perylene
    Indeno[1,2,3-cd]pyrene
    Benzo[b]chrysene (I.S.)
    查看所有结果 (16)

历史批次信息供参考:

分析证书(COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our 文件 section.

如需帮助，请联系 客户支持

已有该产品？

在文件库中查找您最近购买产品的文档。

访问文档库

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系客户支持