技术
LC/MS: suitable
试验参数
column: Titan C18, 10 cm x 2.1 mm I.D., 1.9 μm particles (577124-U)
mobile phase: [A] 0.2 mM ammonium fluoride; [B] methanol
gradient: 40 to 82%B in 4.6 min; to 100%B in 0.05 min; held for 1.15 min; to 40% B in 0.05 min; held for 1.65 min
flow rate: 0.4 mL/min
column temp.: 40 °C
detector: MS, ESI(-), MRM transitions listed on figure
injection: 20 μL
sample: patient serum sample containing estradiol, 56 pmol/L
适用性
application for LC-MS
应用
clinical
分析说明
A fast and robust LC/MS/MS method was developed for the measurement of estradiol in human serum using Titan C18 1.9 μm columns. Ultra high purity LC-MS solvents were used to supply low background interference and low particulate contaminants for robust, trouble-free operation. Cerilliant certified reference material grade standards provided reliable quantification.
法律信息
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持