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Merck
CN

G1006596

HPLC Analysis of Proteins on TSKgel® BioAssist S, Ion Selectivity Comparison

application for HPLC

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技术

HPLC: suitable

试验参数

column: TSKgel BioAssist S, 5 cm x 4.6 mm I.D., 7 μm particles (819686)
mobile phase: [A] 10 mM potassium phosphate, dibasic, pH 7 with phosphoric acid; [B] 0.3 M potassium potassium phosphate, pH 7 with phosphoric acid; ([C] 10 mM sodium phosphate, dibasic, pH 7 with phosphoric acid; [D] 0.3 M sodium potassium phosphate, dibasic, pH 7 with phosphoric acid;)
gradient: 100% A to 100% B (or 100% C to 100% D) in 33 min, held at 100% B (or D) for 5 minutes
flow rate: 0.3 mL/min
pressure: 615 psi total pressure (42 bar total pressure)
column temp.: 25 °C
detector: UV, 280 nm
injection: 5 μL
sample: g/L ea: ribonuclease a, 0.38; cytochrome c, 0.15; lysozyme, 0.23; a-chymptrypsinogen, 0.23

适用性

application for HPLC

应用

life science and biopharma

分析说明

The choice of exchangable counterion can affect retention and selectivity when performing ion exchange chromatography of proteins. This application note compares sodium and potassium gradients for changes in retention and selectivity.

法律信息

TSKgel is a registered trademark of Tosoh Corporation

分析物

说明
    Ribonuclease A from bovine pancreas, Type I-A, powder, ≥60% RNase A basis (SDS-PAGE), ≥50 Kunitz units/mg protein
    α-Chymotrypsinogen A from bovine pancreas, essentially salt-free, lyophilized powder
    Cytochrome c from bovine heart, ≥95% based on Mol. Wt. 12,327 basis, powder, suitable for mammalian cell culture
    Lysozyme from chicken egg white, powder or granules, ≥39,000 units/mg protein

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分析证书(COA)

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