LC/MS Analysis of Nucleosides on Ascentis^® Express F5

Detector: MS, ESI (-) QTOF, EIC, (Pseudouridine m/z 243 and 153; Cytidine m/z 242 and 109; Uridine m/z 243 and 111; 2-Thiocytidine m/z 258 and 126; Guanosine m/z 282 and 150; Inosine m/z 267 and 135; Ribothymidine m/z 257 and 125; 1-Methyladenosine m/z 280 and 148; 2′-O-Methyladenosine m/z 256, 213 and 108; 5-Methylcytidine m/z 256 and 124; 7-Methylguanosine m/z 124 and 164; 3-Methylcytidine methosulfate m/z 256 and 124; N6-Methyladenosine m/z 280 and 148.

Nucleosides are structural subunits of nucleic acids, the macromolecules that convey genetic information in living cells. They contain a molecule of sugar (pentose) bonded to a nitrogen-containing organic ring compound. The nitrogen-containing compound is either a pyrimidine (cytosine, thymine, or uracil) or a purine (adenine or guanine). Nucleosides, derivatives and / or analogues can be used in many different industries and applications as in agrochemistry (herbicides, fungicides and insecticides), therapeutic drugs including a range of antiviral (HIV, hepatitis C virus and herpes simplex) and even antibiotics. This application demonstrates reverse phase interactions with mobile phases that are shown to work well in mass spectrometry conditions.

1-methyladenosine undergoes a Dimroth rearrangement to yield N6-methyladenosine in the standard solution mix.

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