G1007123
UHPLC Analysis of a Rabbit Secretory IgA Monoclonal Antibody on BIOshell A400 Protein C4
application for HPLC
别名:
immunoglobulin, lgA, peptide, protein, reversed-phase, secretory
技术
HPLC: suitable
试验参数
column: BIOshell A400 Protein C4; 10 cm x 2.1 mm I.D., 3.4 μm particles (66825-U)
mobile phase: [A] 0.1% TFA in water; [B] 0.1% TFA in acetonitrile
gradient: [A] 0.1% TFA in water; [B] 0.1% TFA in acetonitrile
flow rate: 0.2 mL/min
pressure: 550 psi (38 bar)
column temp.: 75 °C
detector: UV, 215 nm
injection: 1 μL
sample: Rabbit secretory IgA, 100 μg/mL, 0.05% TFA in water
适用性
application for HPLC
应用
life science and biopharma
分析说明
Secretory immunoglobulin A (IgA) exists as a complex of 2-4 IgA monomers linked through a 15 kDa polypeptide J chain. Rabbit secretory IgA is a 385 kDa antibody composed of two IgA monomers and the J chain. Secretory IgA is resistant to degradative enzymes; therefore, it plays a key role in providing protection against microbes that multiply in bodily secretions. This antibody was chromatographically analysed on a BIOshell A400 Protein C4 column. A prominent shoulder peak was partially resolved from the main analyte peak, indicating the presence of impurities and/or other, post-translationally modified variants.
法律信息
我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.
联系客户支持