Merck
CN
所有图片(1)

文件

G1007615

LC/MS Analysis of Nucleotides on SeQuant® ZIC-cHILIC

application for LC-MS

登录查看公司和协议定价
所有图片(1)

技术

LC/MS: suitable

试验参数

column: SeQuant® ZIC-cHILIC, 10 cm x 2.1 mm, 3.0 μm particles (1.50657)
mobile phase: [A] acetonitrile; [B] 50 mM ammonium acetate, pH 5.0 with acetic acid in water
gradient: 26 to 27% B in 10 min; to 27 to 35% B in 10 min; to 26% B in 0.1 min; hold at 26% B for 9.9 min
flow rate: 0.3 mL/min
pressure: 1029 psi (71 bar)
column temp.: 50 °C
detector: Q-TOF (ESI-negative), XIC of the following m/z values: AMP-346, ADP-426, ATP-506, CMP-322, CDP-402, (CTP-482, GMP-362, GDP-442, GTP-522, UMP-323, UDP-403, UTP-483)
injection: 10 μL
sample: 10-30 μg/mL of nucleotides (AMP, ADP, ATP, CMP, CDP, CTP, GMP, GDP, GTP, UMP, UDP, UTP) (80/20 acetonitrile/50 mM ammonium acetate in water, pH 5.3 with acetic acid)

适用性

application for LC-MS

应用

pharmaceutical (small molecule)

分析说明

Nucleotides are the building blocks of nucleic acids. They comprise a nitrogenous base, a sugar, and a phosphate group making them highly polar and ionic. This causes a challenge for typical reversed phase chromatography (RPC). These charged polar analytes are poorly retained by RPC, and thus typically chromatographed by either ion-exchange chromatography or RPC in conjunction with an ion-pairing reagent. Nucleotide analysis using ion-pair reagents or high concentration of phosphate buffers cause high background and ion source pollution for MS detection. HILIC separation is an alternative that permits sensitive MS detection and without the use of ion-pair reagents.

法律信息

SeQuant is a registered trademark of Merck KGaA, Darmstadt, Germany

分析物

说明
    Adenosine 5′-monophosphate sodium salt, from yeast, ≥99%
    Adenosine 5′-diphosphate sodium salt, bacterial, ≥95% (HPLC)
    Adenosine 5′-triphosphate disodium salt hydrate, BioXtra, ≥99% (HPLC), from microbial
    Cytidine 5′-monophosphate, Sigma Grade, ≥99% (HPLC), synthetic, powder
    Cytidine 5′-diphosphate sodium salt hydrate, from yeast, ≥95%
    Cytidine 5′-triphosphate disodium salt, ~95%
    Guanosine 5′-monophosphate disodium salt hydrate, from yeast, ≥99%
    Guanosine 5′-diphosphate sodium salt, Type I, ≥96% (HPLC)
    Guanosine 5′-triphosphate sodium salt hydrate, ≥95% (HPLC), powder
    Uridine 5′-monophosphate, ≥98%
    Uridine 5′-(trihydrogen diphosphate) sodium salt from Saccharomyces cerevisiae, 95-100%
    Uridine 5′-triphosphate trisodium salt hydrate, from yeast, Type III, ≥96%
    查看所有结果 (12)

分析证书(COA)

输入产品批号来搜索 分析证书(COA) 。批号可以在产品标签上"批“ （Lot或Batch）字后找到。

已有该产品？

为方便起见，与您过往购买产品相关的文件已保存在文档库中。

访问文档库

难以找到您所需的产品或批次号码？

在网站页面上，产品编号会附带包装尺寸/数量一起显示（例如：T1503-25G）。请确保 在“产品编号”字段中仅输入产品编号 (示例: T1503).

示例

T1503
货号
-
25G
包装规格/数量

其它示例：

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

输入内容 1.000309185)

遇到问题？欢迎随时联系我们技术服务 寻求帮助

批号可以在产品标签上"批“ （Lot或Batch）字后面找到。

Aldrich 产品

  • 如果您查询到的批号为 TO09019TO 等，请输入去除前两位字母的批号：09019TO。

  • 如果您查询到的批号含有填充代码（例如05427ES-021），请输入去除填充代码-021的批号：05427ES。

  • 如果您查询到的批号含有填充代码（例如 STBB0728K9），请输入去除填充代码K9的批号：STBB0728。

未找到您寻找的产品？

部分情况下，可能未在线提供COA。如果搜索不到COA，可在线索取。

索取COA

我们的科学家团队拥有各种研究领域经验，包括生命科学、材料科学、化学合成、色谱、分析及许多其他领域.

联系技术服务部门