MISSION^® shRNA Products

Individual MISSION^® shRNA Clones

• Our online tool allows you to view clone-specific validation information and build your own clone sets.
• The individual clones are available in three formats: bacterial glycerol stock, purified plasmid DNA and lentiviral transduction particles
• Locate shRNA clones for your specific gene of interest by clicking 'Search'
• Choose from over 315,000 pre-cloned shRNA constructs for both human and mouse.
• Ordering 25 clones or more? Please click here.

MISSION^® shRNA Gene Family Sets

Choose from 25 Pre-Designed shRNA Gene Panels

• All TRC shRNA clones have been methodically mapped to each current gene set by our Bioinformatics team.
• Gene family sets for human (22) and mice (3) include popular kinases, phosphatases, GPCRs, tyrosine kinases, and epigenetic regulators.
• Any panel can be arrayed in either 96- or 384- well plate formats
• All gene family sets can be used at the benchtop for focused target discovery and pathway analysis.

Create your own panel.
Different gene pathway of interest? Want a more complete list?
Submit a request.

• Gene family sets are fully customizable allowing for complete flexibility to add or delete clone or gene content.

MISSION^® shRNA Libraries

Arrayed genome-wide shRNA libraries

• Most extensive coverage: Pre-cloned genome-wide arrayed libraries contain 245,000+ clone shRNA library targeting 20,000+ human and 21,000+ mouse genes.
• High-throughput manufacturing: World class high-throughput viral manufacturing.
• Flexible format options: Entire libraries or single gene sets offered in glycerol stock, DNA, or viral format.
• Extended Coverage=Average of 5 - 6 clones targeting each gene.

Pooled shRNA Libraries
Whole genome shRNA screening for a fraction of the cost.

• Affordable - Bench top discovery without robotics or liquid handling.
• Comprehensive - Trusted TRC (The RNAi Consortium) content with shRNA taregting 20,000+ human or 21,000+ mouse genes including TRC 1.5 and 2.0 (unique and validated) add-ons which are exclusive to Sigma.
• Universal - Simplified whole genome RNAi screening in standard or difficult to expand cell lines.
• Versatile - Compatible with FACS and most downstream fluorescent assays.

Custom MISSION^® shRNA

Fully customizable shRNA sequence, vector, and lentiviral manufacturing

• Select by methods other than puromycin, including neomycin (G418) and FACS via reporters.
• Confirm your lentiviral delivery through a fluorescent reporter.
• Customize your shRNA sequence.
• Eliminate tedious cloning and viral production.
• Service includes: shRNA design, cloning, sequence verifications, DNA quantitation, and titer determination.
  
  Custom Capabilities: Bringing you more flexibility
• Contract manufacturing: Send us your clones for lentiviral production! We accommodates high-throughput projects, private collections, and alternate libraries. Please request a quote or contact if interested.

Create Your Own shRNA Pool

Focus on the genes essential to your research by creating your own custom shRNA pool

• Create your custom shRNA pool for maximum return of relevant hits
• Customize your volume and aliquoting needs to further enhance your ability to rapidly screen multiple cell lines.
• Titers and volumes adequate for in vitro, in vivo and xenograft applications.
• Partner with Sigma's next-generation sequencing services for data deconvolution and maximum data return from your screening efforts.

Services

Deconvolution of shRNA Pools

• Sigma's deconvolution service lets you easily identify the genes that impact your pooled shRNA screen.
• Next-generation sequencing of clones gives a precise number of individual clone occurrence within a pooled shRNA sample.
• Comprehensive, reproducible results from pooled shRNA screens.
• Statistically robust and information-rich data.

shRNA Controls

Measure transduction and knockdown efficiency, or monitor off-target effects.

• Positive Transduction Controls: Empty Vector, TurboGFP, TagCFP, TagYFP, TagRFP, and TagFP365 Controls
• Positive Knockdown Controls: TurboGFP, eGFP, luciferase, B2M, and ARHGDIA controls
• Negative Controls: Empty Vector, Non-target, Turbo GFP, eGFP, and luciferase controls

Delivery

Produce high-titer lentivirus using the MISSION^® Lentiviral Packaging Mix

• Accelerate viral production by skipping tedious but critical multivariable plasmid optimization.
• Produce high titer lentivirus with MISSION^® shRNA plasmids with the included easy to use protocol.
• Safely produce lentivirus utilizing the three plasmid system with self inactivating, long terminal repeat (SIN/LTR) regions.
• Choose between small or large scale lentivirus production with our conveniently sized 250 µl or 1.7 ml sizes.

Inducible MISSION^® shRNA

Tight regulation meets great gene silencing

• IPTG-inducible shRNA pLKO-vectors allow for fast response and temporally-controlled gene silencing useful for essential or lethal gene knockdown.
• Proven to work in vivo and in vitro
• Single vector systems in 1X LacO and 3X LacO formats for effective inducible knockdown
• Available on demand service includes: shRNA design(choose any TRC clone or custom sequence), cloning, sequence verification, DNA quantitation, and titer determination.

Targeted Integration shRNA

MISSION^® Taregeted Integration shRNA kit, Powerd by CompoZr^® Zinc Finger Technology.

• Validated pair of zinc finger nucleases (ZFNs) engineered to target AAVS1 locus enables efficient targeted integration of shRNA encoding sequence
• Consistent site for transgene delivery as AAVS1 site is likely present in every human cell line
• No random integration leads to better control of target gene expression
• Effective gene knockdown comparable to lentiviral shRNA constructs