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Thomas K Karikari et al.
ACS chemical neuroscience, 11(19), 3117-3129 (2020-08-25)
Tau neurofibrillary tangles are key pathological features of Alzheimer's disease and other tauopathies. Recombinant protein technology is vital for studying the structure and function of tau in physiology and aggregation in pathophysiology. However, open-source and well-characterized plasmids for efficiently expressing
Jianghong Zhong et al.
Communications biology, 4(1), 865-865 (2021-07-15)
A single-nucleotide polymorphism of neutrophil cytosolic factor 1 (Ncf1), leading to an impaired generation of reactive oxygen species (ROS), is a causative genetic factor for autoimmune disease. To study a possible tumor protection effect by the Ncf1 mutation in a
Mohamed A Abu El Maaty et al.
Science advances, 7(31) (2021-08-01)
Epidemiological data have linked vitamin D deficiency to the onset and severity of various cancers, including prostate cancer, and although in vitro studies have demonstrated anticancer activities for vitamin D, clinical trials provided conflicting results. To determine the impact of
Sophia Hsin-Jung Li et al.
Nature protocols, 16(6), 2802-2825 (2021-05-07)
Several essential components of the electron transport chain, the major producer of ATP in mammalian cells, are encoded in the mitochondrial genome. These 13 proteins are translated within mitochondria by 'mitoribosomes'. Defective mitochondrial translation underlies multiple inborn errors of metabolism
Kelsey A Haugh et al.
eLife, 10 (2021-07-06)
Early events in retrovirus transmission are determined by interactions between incoming viruses and frontline cells near entry sites. Despite their importance for retroviral pathogenesis, very little is known about these events. We developed a bioluminescence imaging (BLI)-guided multiscale imaging approach
Jianqing Liang et al.
Advanced science (Weinheim, Baden-Wurttemberg, Germany), 9(9), e2105568-e2105568 (2022-01-23)
Euthyroidism is of profound importance for lifetime health. However, the early diagnosis or therapeutics of thyroid developmental defects has not been established, mainly due to limited understanding of human thyroid development and a lack of recapitulating research model. Herein, the
Ramón Cervantes-Rivera et al.
Bio-protocol, 10(18), e3757-e3757 (2021-03-05)
Gene transcription in bacteria often starts some nucleotides upstream of the start codon. Identifying the specific Transcriptional Start Site (TSS) is essential for genetic manipulation, as in many cases upstream of the start codon there are sequence elements that are
Emma R Stewart et al.
Methods in molecular biology (Clifton, N.J.), 1861, 103-112 (2018-09-16)
Immunodetection of nuclear antigens is often complicated by epitope masking, so that proteins known to function in the nucleus are sometimes not easily detected at their sites of action. Moreover, protein populations that are detected before unmasking can be very
Francisco J Calero-Cuenca et al.
Current biology : CB, 31(7), 1521-1530 (2021-02-11)
Cells actively position their nuclei within the cytoplasm for multiple cellular and physiological functions.1-3 Consequently, nuclear mispositioning is usually associated with cell dysfunction and disease, from muscular disorders to cancer metastasis.4-7 Different cell types position their nuclei away from the
Rok Razpotnik et al.
International journal of molecular sciences, 22(14) (2021-07-25)
Circular RNAs (circRNAs) are increasingly recognized as having a role in cancer development. Their expression is modified in numerous cancers, including hepatocellular carcinoma (HCC); however, little is known about the mechanisms of their regulation. The aim of this study was
Prasad D Trivedi et al.
Molecular therapy. Methods & clinical development, 24, 154-170 (2022-01-25)
Recent clinical successes have propelled recombinant adeno-associated virus vectors (rAAV) to the center stage for human gene therapy applications. However, the exploding demand for high titers of highly pure rAAV vectors for clinical applications and market needs remains hindered by
Karin Purshouse et al.
eLife, 11 (2022-12-09)
Extrachromosomal DNA (ecDNA) are frequently observed in human cancers and are responsible for high levels of oncogene expression. In glioblastoma (GBM), ecDNA copy number correlates with poor prognosis. It is hypothesized that their copy number, size, and chromatin accessibility facilitate
David Brown et al.
Frontiers in immunology, 12, 730825-730825 (2021-11-12)
Engineered variants of recombinant adeno-associated viruses (rAAVs) are being developed rapidly to meet the need for gene-therapy delivery vehicles with particular cell-type and tissue tropisms. While high-throughput AAV engineering and selection methods have generated numerous variants, subsequent tropism and response
Bernard K van der Veer et al.
Nucleic acids research, 51(11), 5469-5498 (2023-04-07)
Gastrulation begins when the epiblast forms the primitive streak or becomes definitive ectoderm. During this lineage bifurcation, the DNA dioxygenase TET1 has bipartite functions in transcriptional activation and repression, but the mechanisms remain unclear. By converting mouse embryonic stem cells
Evgeniya Trofimenko et al.
eLife, 10 (2021-10-30)
Cell-penetrating peptides (CPPs) allow intracellular delivery of bioactive cargo molecules. The mechanisms allowing CPPs to enter cells are ill-defined. Using a CRISPR/Cas9-based screening, we discovered that KCNQ5, KCNN4, and KCNK5 potassium channels positively modulate cationic CPP direct translocation into cells
André Felipe Rodrigues et al.
Clinical science (London, England : 1979), 135(11), 1353-1367 (2021-05-21)
In spite of the fact that the modulatory effects of angiotensin II (Ang II) on the sympathetic nerve activity to targeted organs involved in blood pressure (BP) regulation is well acknowledged, the local production of this peptide in the brain
Zila Martinez-Lozada et al.
Neurochemistry international, 139, 104787-104787 (2020-07-11)
Astrocytes have diverse functions that are supported by their anatomic localization between neurons and blood vessels. One of these functions is the clearance of extracellular glutamate. Astrocytes clear glutamate using two Na+-dependent glutamate transporters, GLT-1 (also called EAAT2) and GLAST
Shenli Yuan et al.
Cell discovery, 9(1), 13-13 (2023-01-31)
Although parental genomes undergo extensive epigenetic reprogramming to be equalized after fertilization, whether they play different roles in human zygotic genome activation (ZGA) remains unknown. Here, we mapped parental transcriptomes by using human parthenogenetic (PG) and androgenetic (AG) embryos during
Qiang Li et al.
Frontiers in oncology, 11, 619915-619915 (2021-04-13)
The long noncoding RNA (lncRNA) LINC00152, also known as CYTOR, displays aberrant expression in various cancers. However, its clinical value and functional mechanisms in breast cancer remain insufficiently understood. Our study found that LINC00152 is significantly upregulated in breast cancer
Guillaume Beaudoin-Bussières et al.
Cell reports, 38(7), 110368-110368 (2022-02-07)
Emerging evidence indicates that both neutralizing and Fc-mediated effector functions of antibodies contribute to protection against SARS-CoV-2. It is unclear whether Fc-effector functions alone can protect against SARS-CoV-2. Here, we isolated CV3-13, a non-neutralizing antibody, from a convalescent individual with
Kornkamon Lertsuwan et al.
The Prostate, 77(7), 729-742 (2017-02-09)
Previous studies showed that human bone marrow stromal HS-5 cells secreted unidentified factor(s) inducing PCa cell death. Herein, the HS-5-derived factor (HS-5 DF) was characterized and identified. Conditioned media from confluent HS-5 cells were collected and modified for biochemical characteristic
Mariana Bresque et al.
The Journal of biological chemistry, 298(3), 101711-101711 (2022-02-13)
Acute and chronic inflammations are key homeostatic events in health and disease. Sirtuins (SIRTs), a family of NAD-dependent protein deacylases, play a pivotal role in the regulation of these inflammatory responses. Indeed, SIRTs have anti-inflammatory effects through a myriad of
Marisa Müller et al.
Bio-protocol, 11(5), e3946-e3946 (2021-04-03)
RNA-protein interactions are often mediated by dedicated canonical RNA binding domains. However, interactions through non-canonical domains with unknown specificity are increasingly observed, raising the question how RNA targets are recognized. Knowledge of the intrinsic RNA binding specificity contributes to the
Wenli Zhang et al.
Methods in molecular biology (Clifton, N.J.), 1284, 71-89 (2015-03-12)
Genomic regions associated with regulatory proteins are known to be highly sensitive to DNase I digestion and are termed DNase I hypersensitive sites (DHSs). DHSs can be identified by DNase I digestion followed by high-throughput DNA sequencing (DNase-seq). DNase-seq has
Kirsten Richter et al.
The Journal of biological chemistry, 295(23), 7849-7864 (2020-04-23)
Activation of the T cell receptor (TCR) results in binding of the adapter protein Nck (noncatalytic region of tyrosine kinase) to the CD3ϵ subunit of the TCR. The interaction was suggested to be important for the amplification of TCR signals
Paolo Fattorini et al.
International journal of molecular sciences, 21(20) (2020-10-18)
RNA isolated from fixed and paraffin-embedded tissues is widely used in biomedical research and molecular pathology for diagnosis. In the present study, we have set-up a method based on high performance liquid chromatography (HPLC) to investigate the effects of different
Christina N Cheng et al.
Journal of visualized experiments : JoVE, (89)(89), doi:10-doi:10 (2014-08-01)
The zebrafish embryo is now commonly used for basic and biomedical research to investigate the genetic control of developmental processes and to model congenital abnormalities. During the first day of life, the zebrafish embryo progresses through many developmental stages including
Joana G Marques et al.
eLife, 9 (2020-08-04)
The NuRD complex subunit CHD4 is essential for fusion-positive rhabdomyosarcoma (FP-RMS) survival, but the mechanisms underlying this dependency are not understood. Here, a NuRD-specific CRISPR screen demonstrates that FP-RMS is particularly sensitive to CHD4 amongst the NuRD members. Mechanistically, NuRD
Yohey Ogawa et al.
Scientific reports, 11(1), 17340-17340 (2021-09-01)
Vertebrate photoreceptors are categorized into two broad classes, rods and cones, responsible for dim- and bright-light vision, respectively. While many molecular features that distinguish rods and cones are known, gene expression differences among cone subtypes remain poorly understood. Teleost fishes
In Kyoung Mah et al.
Developmental biology, 416(1), 82-97 (2016-06-18)
Polarized epithelia define boundaries, spaces, and cavities within organisms. Cavitation, a process by which multicellular hollow balls or tubes are produced, is typically associated with the formation of organized epithelia. In order for these epithelial layers to form, cells must
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