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显示 1-30 #N/A 143 条结果 关于 "08-115" 范围 论文
Paulina Moreno-Layseca et al.
Nature cell biology, 23(10), 1073-1084 (2021-10-08)
Spatially controlled, cargo-specific endocytosis is essential for development, tissue homeostasis and cancer invasion. Unlike cargo-specific clathrin-mediated endocytosis, the clathrin- and dynamin-independent endocytic pathway (CLIC-GEEC, CG pathway) is considered a bulk internalization route for the fluid phase, glycosylated membrane proteins and
Laurent Taupenot
Current protocols in cell biology, Chapter 15, Unit 15-Unit 15 (2008-01-30)
The catecholamine-secreting PC12 cell line derived from the rat adrenal medulla has long been considered a model system for neurosecretion and neuronal differentiation. PC12 cells contain a large number of secretory granules (otherwise known as large dense-core vesicles) for storage
Dipali Goyal et al.
International journal of molecular sciences, 24(8) (2023-04-28)
Neovascularization is an essential process in organismal development and aging. With aging, from fetal to adult life, there is a significant reduction in neovascularization potential. However, the pathways which play a role in increased neovascularization potential during fetal life are
Hui Liu et al.
Biological procedures online, 22, 3-3 (2020-02-06)
Cell invasion through extracellular matrix (ECM) is a critical step in tumor metastasis. To study cell invasion in vitro, the internal microenvironment can be simulated via the application of 3D models. This study presents a method for 3D invasion examination
Wafaa Alabsi et al.
Pharmaceutics, 13(8) (2021-08-29)
The peptide hormone Angiotensin (1-7), Ang (1-7) or (Asp-Arg-Val-Tyr-Ile-His-Pro), is an essential component of the renin-angiotensin system (RAS) peripherally and is an agonist of the Mas receptor centrally. Activation of this receptor in the CNS stimulates various biological activities that
C Fink et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 14(5), 669-679 (2000-04-01)
To examine the influence of chronic mechanical stretch on functional behavior of cardiac myocytes, we reconstituted embryonic chick or neonatal rat cardiac myocytes to a 3-dimensional engineered heart tissue (EHT) by mixing freshly isolated cells with neutralized collagen I and
Shemona Rattila et al.
Reproduction (Cambridge, England), 160(5), 737-750 (2020-10-17)
We previously reported that binding to heparan sulfate (HS) is required for the ability of the placentally secreted pregnancy-specific glycoprotein 1 (PSG1) to induce endothelial tubulogenesis. PSG1 is composed of four immunoglobulin-like domains but which domains of the protein bind
Alexander Brown et al.
Biomaterials, 243, 119921-119921 (2020-03-17)
In vitro tissue engineered models are poised to have significant impact on disease modeling and preclinical drug development. Reliable methods to induce microvascular networks in such microphysiological systems are needed to improve the size and physiological function of these models.
Rania Harati et al.
PloS one, 17(1), e0262152-e0262152 (2022-01-14)
The brain endothelial barrier permeability is governed by tight and adherens junction protein complexes that restrict paracellular permeability at the blood-brain barrier (BBB). Dysfunction of the inter-endothelial junctions has been implicated in neurological disorders such as multiple sclerosis, stroke and
Kimberley A Mander et al.
Journal of veterinary diagnostic investigation : official publication of the American Association of Veterinary Laboratory Diagnosticians, Inc, 32(2), 277-281 (2019-10-15)
Clostridium perfringens type D epsilon toxin (ETX) is responsible for a severe and frequently fatal neurologic disorder in ruminant livestock. Light microscopic, immunohistochemical, and ultrastructural studies have suggested that ETX injury to the cerebral microvasculature, with subsequent severe, generalized vasogenic
The insulin receptor regulates the persistence of mechanical nociceptive sensitization in flies and mice.
Wang, et al.
Biology Open, 12 (2023)
Yichen Cheng et al.
Cell reports, 39(9), 110885-110885 (2022-06-02)
Physiological blood-tissue barriers play a critical role in separating the circulation from immune-privileged sites and denying access to blood-borne viruses. The mechanism of virus restriction by these barriers is poorly understood. We utilize induced pluripotent stem cell (iPSC)-derived human brain
Aastha Kapoor et al.
Matrix biology plus, 6-7, 100025-100025 (2021-02-06)
We present a simplified method for conducting aortic ring assays which yields robust sprouting and high reproducibility targeted towards matrix biologists studying angiogenesis and extracellular matrix signaling. Main adjustments from previously established protocols include embedding aortic rings between two layers
William M Yarbrough et al.
The Journal of thoracic and cardiovascular surgery, 143(1), 215-223 (2011-11-08)
Patients with severe left ventricular pressure overload secondary to aortic stenosis can present with signs and symptoms of heart failure despite normal left ventricular ejection fraction. This process occurs, at least in part, as a result of left ventricular pressure
Jack Campbell et al.
ACS applied materials & interfaces, 13(2), 3259-3269 (2021-01-08)
The polymer layer-by-layer assembly is accounted among the most attractive approaches for the design of advanced drug delivery platforms and biomimetic materials in 2D and 3D. The multilayer capsules can be made of synthetic or biologically relevant (e.g., natural) polymers.
Loïc Binan et al.
Bio-protocol, 9(22), e3428-e3428 (2019-11-20)
Capturing single cells from large heterogenous populations based solely on observable traits is necessary for many cell biology applications and remains a major technical challenge. The protocol we present allows the isolation of viable and metabolically active cells selected for
Sejin Chung et al.
Clinical cancer research : an official journal of the American Association for Cancer Research, 25(7), 2290-2304 (2018-12-13)
Cancer cells often have deficiencies in cell-cycle control mechanisms and could be dependent on specific cell-cycle checkpoints to maintain viability. Because of the documented role of KRAS in driving replication stress, we targeted the checkpoint governing DNA replication using CHK1
H Uyttendaele et al.
Developmental biology, 196(2), 204-217 (1998-05-13)
Elongation and branching of epithelial ducts is a crucial event during the development of the mammary gland. Branching morphogenesis of the mouse mammary epithelial TAC-2 cell line was used as an assay to examine the role of Wnt, HGF, TGF-beta
Ren Song et al.
Molecular therapy. Methods & clinical development, 27, 73-88 (2022-10-04)
A limitation for recombinant adeno-associated virus (rAAV)-mediated gene transfer into the central nervous system (CNS) is the low penetration of vectors across the human blood-brain barrier (BBB). High doses of intravenously delivered vector are required to reach the CNS, which
Chiara Zambarda et al.
European journal of cell biology, 101(4), 151274-151274 (2022-09-25)
Several factors present in the extracellular environment regulate epithelial cell adhesion and dynamics. Among them, growth factors such as EGF, upon binding to their receptors at the cell surface, get internalized and directly activate the acto-myosin machinery. In this study
Alberto Pappalardo et al.
Advances in wound care, 10(9), 490-502 (2020-09-02)
Objective: We compared the capability of human umbilical vein endothelial cells (HUVECs), induced pluripotent stem cell (iPSC)-derived endothelial cells (iECs), and human dermal blood endothelial cells (HDBECs) to effectively vascularize engineered human skin constructs (HSCs) in vitro and on immunodeficient
Qiuyang Zhang et al.
American journal of physiology. Gastrointestinal and liver physiology, 322(6), G598-G614 (2022-04-06)
The pathogenesis of subsquamous intestinal metaplasia (SSIM), in which glands of Barrett's esophagus (BE) are buried under esophageal squamous epithelium, is unknown. In a rat model of reflux esophagitis, we found that columnar-lined esophagus developed via a wound-healing process involving
Jia Sun et al.
Redox biology, 39, 101811-101811 (2020-12-29)
Vascular complications of diabetes are a serious challenge in clinical practice, and effective treatments are an unmet clinical need. Acidic fibroblast growth factor (aFGF) has potent anti-oxidative properties and therefore has become a research focus for the treatment of diabetic
Esther T Stoeckli et al.
Current protocols in cell biology, 61, 9-9 (2014-02-11)
Cell-cell adhesion is a fundamental requirement for all multicellular organisms. The calcium-independent cell adhesion molecules of the immunoglobulin superfamily (IgSF-CAMs) represent a major subgroup. They consist of immunoglobulin folds alone or in combination with other protein modules, often fibronectin type-III
Meng-Yin Yang et al.
Journal of biomedical materials research. Part B, Applied biomaterials, 103(3), 618-628 (2014-06-27)
Three-dimensional (3-D) tissue engineered constructs provide a platform for examining how the local extracellular matrix contributes to the malignancy of various cancers, including human glioblastoma multiforme. Here, we describe a simple and innovative 3-D culture environment and assess its potential
Erik S Knudsen et al.
Gut, 70(1), 127-138 (2020-05-20)
This study exploits the intersection between molecular-targeted therapies and immune-checkpoint inhibition to define new means to treat pancreatic cancer. Patient-derived cell lines and xenograft models were used to define the response to CDK4/6 and MEK inhibition in the tumour compartment.
Carmen Gallego et al.
Frontiers in cellular and infection microbiology, 13, 1138232-1138232 (2023-06-01)
Human papillomaviruses (HPVs) are highly prevalent commensal viruses that require epithelial stratification to complete their replicative cycle. While HPV infections are most often asymptomatic, certain HPV types can cause lesions, that are usually benign. In rare cases, these infections may
Detailed Molecular Mechanism and Potential Drugs for COL1A1 in Carboplatin-Resistant Ovarian Cancer.
Feng Yang et al.
Frontiers in oncology, 10, 576565-576565 (2021-03-09)
Carboplatin resistance in ovarian cancer (OV) is a major medical problem. Thus, there is an urgent need to find novel therapeutic targets to improve the prognosis of patients with carboplatin-resistant OV. Accumulating evidence indicates that the gene COL1A1 (collagen type
J J Tomasek et al.
The Journal of biological chemistry, 272(11), 7482-7487 (1997-03-14)
Gelatinase A (GL-A) is a matrix metalloproteinase (MMP) involved in both connective tissue remodeling and tumor invasion. GL-A activation is mediated by a membrane-type MMP (MT-MMP) that cleaves the GL-A propeptide. In this study, we examined the role of the
Mark D Bunting et al.
Science advances, 8(11), eabk3327-eabk3327 (2022-03-17)
Natural killer (NK) cells reject major histocompatibility complex class I (MHC-I)-deficient bone marrow through direct cytotoxicity but not solid organ transplants devoid of MHC-I. Here, we demonstrate an immediate switch in NK cell function upon exit from the circulation, characterized
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