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Yuan-Na Lin et al.
Oncoimmunology, 11(1), 2027136-2027136 (2022-02-08)
Pancreatic cancer remains largely unresponsive to immune modulatory therapy attributable in part to an immunosuppressive, desmoplastic tumor microenvironment. Here, we analyze mechanisms of cancer cell-autonomous resistance to T cells. We used a 3D co-culture model of cancer cell spheroids from
Jeremy S Logue et al.
Oncogene, 37(16), 2104-2121 (2018-01-31)
Cancer cell migration requires that cells respond and adapt to their surroundings. In the absence of extracellular matrix cues, cancer cells will undergo a mesenchymal to ameboid transition, whereas a highly confining space will trigger a switch to "leader bleb-based"
Martin Kolev et al.
Immunity, 52(3), 513-527 (2020-03-19)
Intrinsic complement C3 activity is integral to human T helper type 1 (Th1) and cytotoxic T cell responses. Increased or decreased intracellular C3 results in autoimmunity and infections, respectively. The mechanisms regulating intracellular C3 expression remain undefined. We identified complement, including
Fengming Liu et al.
Frontiers in cellular and infection microbiology, 11, 701278-701278 (2021-07-27)
SARS-CoV-2 infection can cause fatal inflammatory lung pathology, including thrombosis and increased pulmonary vascular permeability leading to edema and hemorrhage. In addition to the lung, cytokine storm-induced inflammatory cascade also affects other organs. SARS-CoV-2 infection-related vascular inflammation is characterized by
Nathan Criem et al.
Scientific reports, 8(1), 4722-4722 (2018-03-20)
Atrioventricular septal defects often result from impaired endocardial cushion development. Endothelial-to-mesenchymal transition (EndoMT) is a critical event in endocardial cushion development that initiates in the atrioventricular canal (AVC). In ex vivo EndoMT studies, mouse AVCs are flat-mounted on a collagen
Berin Upcin et al.
Cells, 10(7) (2021-08-08)
Blocking tumor vascularization has not yet come to fruition to the extent it was hoped for, as angiogenesis inhibitors have shown only partial success in the clinic. We hypothesized that under-appreciated vascular wall-resident stem and progenitor cells (VW-SPCs) might be
Jennifer L Lucitti et al.
American journal of physiology. Heart and circulatory physiology, 309(7), H1141-H1150 (2015-08-16)
The number and diameter of native collaterals in tissues of healthy mice vary widely, resulting in large differences in tissue injury in occlusive diseases. Recent studies suggest similar variation may exist in humans. Collateral variation in mice is determined by
Asha M Das et al.
Nature protocols, 10(6), 904-915 (2015-05-23)
Cell migration is a key feature of virtually every biological process, and it can be studied in a variety of ways. Here we outline a protocol for the in vitro study of cell migration using a ring barrier-based assay. A
Athina-Myrto Chioni et al.
Methods in molecular biology (Clifton, N.J.), 1487, 255-267 (2016-12-08)
Organotypic models are 3D in vitro representations of an in vivo environment. Their complexity can range from an epidermal replica to the establishment of a cancer microenvironment. These models have been used for many years, in an attempt to mimic
Siguang Xu et al.
Molecular cancer therapeutics, 12(8), 1665-1675 (2013-05-22)
Basal-like breast cancer is an aggressive disease for which targeted therapies are lacking. Recent studies showed that basal-like breast cancer is frequently associated with an increased activity of the phosphatidylinositol 3-kinase (PI3K) pathway, which is critical for cell growth, survival
Simone Barbero et al.
Cancer research, 69(9), 3755-3763 (2009-04-23)
Caspase-8 is a proapoptotic protease that suppresses neuroblastoma metastasis by inducing programmed cell death. Paradoxically, caspase-8 can also promote cell migration among nonapoptotic cells; here, we show that caspase-8 can promote metastasis when apoptosis is compromised. Migration is enhanced by
Jermaine Goveia et al.
Cancer cell, 37(1), 21-36 (2020-01-15)
Heterogeneity of lung tumor endothelial cell (TEC) phenotypes across patients, species (human/mouse), and models (in vivo/in vitro) remains poorly inventoried at the single-cell level. We single-cell RNA (scRNA)-sequenced 56,771 endothelial cells from human/mouse (peri)-tumoral lung and cultured human lung TECs, and detected
Deron R Herr et al.
Scientific reports, 6, 24541-24541 (2016-04-16)
Ototoxic drugs, such as platinum-based chemotherapeutics, often lead to permanent hearing loss through apoptosis of neuroepithelial hair cells and afferent neurons of the cochlea. There is no approved therapy for preventing or reversing this process. Our previous studies identified a
Ignacio Fernando Hall et al.
EMBO molecular medicine, 13(10), e14060-e14060 (2021-09-23)
The role of single nucleotide polymorphisms (SNPs) in the etiopathogenesis of cardiovascular diseases is well known. The effect of SNPs on disease predisposition has been established not only for protein coding genes but also for genes encoding microRNAs (miRNAs). The
Da Li et al.
Nature communications, 11(1), 342-342 (2020-01-19)
Precise control of hepatic glucose production (HGP) is pivotal to maintain systemic glucose homeostasis. HNF4α functions to stimulate transcription of key gluconeogenic genes. HNF4α harbors two promoters (P2 and P1) thought to be primarily active in fetal and adult livers
David A Proia et al.
Nature protocols, 1(1), 206-214 (2007-04-05)
Establishing a model system that more accurately recapitulates both normal and neoplastic breast epithelial development in rodents is central to studying human breast carcinogenesis. However, the inability of human breast epithelial cells to colonize mouse mammary fat pads is problematic.
Alexander Brown et al.
Biomaterials, 243, 119921-119921 (2020-03-17)
In vitro tissue engineered models are poised to have significant impact on disease modeling and preclinical drug development. Reliable methods to induce microvascular networks in such microphysiological systems are needed to improve the size and physiological function of these models.
Aastha Kapoor et al.
Matrix biology plus, 6-7, 100025-100025 (2021-02-06)
We present a simplified method for conducting aortic ring assays which yields robust sprouting and high reproducibility targeted towards matrix biologists studying angiogenesis and extracellular matrix signaling. Main adjustments from previously established protocols include embedding aortic rings between two layers
Jack Campbell et al.
ACS applied materials & interfaces, 13(2), 3259-3269 (2021-01-08)
The polymer layer-by-layer assembly is accounted among the most attractive approaches for the design of advanced drug delivery platforms and biomimetic materials in 2D and 3D. The multilayer capsules can be made of synthetic or biologically relevant (e.g., natural) polymers.
William M Yarbrough et al.
The Journal of thoracic and cardiovascular surgery, 143(1), 215-223 (2011-11-08)
Patients with severe left ventricular pressure overload secondary to aortic stenosis can present with signs and symptoms of heart failure despite normal left ventricular ejection fraction. This process occurs, at least in part, as a result of left ventricular pressure
Qiaowei Ngo et al.
Wound repair and regeneration : official publication of the Wound Healing Society [and] the European Tissue Repair Society, 27(6), 715-719 (2019-07-06)
Evaluating interactions between dressing and wound is important for understanding wound management. This study quantitatively compared four polyurethane foam-based wound dressings for their absorption profile, cell penetration, and adherence using two novel in vitro assays. The dressing with uniform pore
Nathan Criem et al.
Scientific reports, 10(1), 5946-5946 (2020-04-02)
An amendment to this paper has been published and can be accessed via a link at the top of the paper.
Gareth W Fearnley et al.
Biology open, 8(5) (2019-05-11)
New blood vessel sprouting (angiogenesis) and vascular physiology are fundamental features of metazoan species but we do not fully understand how signal transduction pathways regulate diverse vascular responses. The vascular endothelial growth factor (VEGF) family bind membrane-bound receptor tyrosine kinases
Odeta Meçe et al.
Nature communications, 13(1), 2760-2760 (2022-05-20)
Autophagy has vasculoprotective roles, but whether and how it regulates lymphatic endothelial cells (LEC) homeostasis and lymphangiogenesis is unknown. Here, we show that genetic deficiency of autophagy in LEC impairs responses to VEGF-C and injury-driven corneal lymphangiogenesis. Autophagy loss in
Marianne Baker et al.
Nature protocols, 7(1), 89-104 (2011-12-24)
Here we provide a protocol for quantitative three-dimensional ex vivo mouse aortic ring angiogenesis assays, in which developing microvessels undergo many key features of angiogenesis over a timescale similar to that observed in vivo. The aortic ring assay allows analysis
Caroline Chagnot et al.
PloS one, 8(3), e59386-e59386 (2013-03-22)
Enterohemorrhagic Escherichia coli (EHEC) O157:H7 are responsible for repeated food-poisoning cases often caused by contaminated burgers. EHEC infection is predominantly a pediatric illness, which can lead to life-threatening diseases. Ruminants are the main natural reservoir for EHEC and food contamination
Shinji Iizuka et al.
European journal of cell biology, 99(7), 151122-151122 (2020-10-19)
The scaffold protein Tks5α is required for invadopodia-mediated cancer invasion both in vitro and in vivo. We have previously also revealed a role for Tks5 in tumor cell growth using three-dimensional (3D) culture model systems and mouse transplantation experiments. Here
Neha Pincha et al.
Bio-protocol, 8(18), e3021-e3021 (2018-09-20)
The collagen contraction assay is an in vitro, three-dimensional method to determine the factor(s) affecting the contractile behavior of activated cells such as fibroblasts in either physiological or pathological scenarios. The collagen lattices/hydrogels are seeded with fibroblasts to mimic the
Chao Niu et al.
Autophagy, 15(5), 843-870 (2019-01-18)
Studies regarding macroautophagic/autophagic regulation in endothelial cells (ECs) under diabetic conditions are very limited. Clinical evidence establishes an endothelial protective effect of metformin, but the underlying mechanisms remain unclear. We aimed to investigate whether metformin exerts its protective role against
Rahimeh B Attasgah et al.
iScience, 25(4), 104019-104019 (2022-03-29)
Hybrid hydrogels composed of chitosan (CS) and hyaluronic acid (HA) and collagen (Coll) were prepared by polyelectrolyte complex self-assembly. These scaffolds displayed a good intermingling of the polymeric chains, with porosities above 80% and good interconnected structures with pore sizes
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