9471 Buchs / Switzerland
Tel. +41 / 81 755 25 11 · Fax +41 / 81 756 54
General procedure for labelling proteins with maleimides
1) Dissolve the protein at 50–100 µM in
cortex
and hippocampus of GAP43-/- mice relative to wild-type
(WT) controls.4
Phosphorylation by PKC at serine 41 correlates with
nerve-terminal sprouting and long-term potentiation
since high
be prolonged up to 12 h at 4°C.
Separation of conjugates from free dye
The purification set (D, spin column, wash tube and collection tube) is designed for 100 µl volume capacity
containing
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Fax: ++41 81 755 2815
Page 2 / 3
3. Transfer the protein solution to the vial of reactive dye.
4. Incubate the reaction mixture for 2 hours while gently
E-mail: Flukatec@europe.sial.com
Fax: ++41 81 755 2815
Page 2 / 3
3. Transfer the protein solution to the vial of reactive dye.
4. Incubate the reaction mixture for 2 hours while gently
productivity
Cellvento™ CHO-100 cell culture media
Superior performance
in cell growth and productivity.
w305101_pharmail_0312_v8_Einzelseiten.indd 5 21.11.12 11:41
6
To reduce
andand
ISO G
uide 3
4
ISO G
uide 3
4
Certificate page 2 of 3 Sigma-Aldrich Production GmbH, Industriestrasse 25, 9471 Buchs/Switzerland, Tel +41-81-755-2511, Fax +41-81-756-5449, e-mail: