proteins allows for increased (4-5 fold) amounts
of serum that can be loaded onto a gel (data not shown).
ProteoPrep Blue typically removes >95% human albumin
and >80% human IgG from 75 ml serum which allows for
been tested. It is expected that the antibody
may also react with mouse (73/75), bovine (71/75), human (69/75) and rabbit (64/75) due to
sequence homology.
FORMAT: Affinity purified immunoglobulin
Concentrate
W3145 100 mL 200 mL
Elution
Solution
E5536 25 mL 125 mL
Binding Plate B8938 1 each 4 each
Wash Plate W3270 1 each 4 each
Elution Plate,
U-Bottom
E5911 1 each 4 each
to contact inhibition.
3. Gently aspirate medium from the flask.
4. Pipet 10 ml fresh medium, pre-warmed to 28oC, into the T-75 flask.
5. Use a sterile scraper or repeated pipetting to gently dislodge
Valve, and Reservoir
5.2 bar (75 psi)
5.2 bar (75 psi)
5.2 bar (75 psi)
3.8 bar (55 psi)
Working temperature range 4–40 °C (39–104 °F)
Capacity 800 mL
Holdup volume 4 mL
Dimensions width/diameter length
Concentrate
W3145 100 mL 200 mL
Elution
Solution
E5536 25 mL 125 mL
Binding Plate B8938 1 each 4 each
Wash Plate W3270 1 each 4 each
Elution Plate,
U-Bottom
E5911 1 each 4 each
Sample Standard
Incubation solution 150 �l 150 �l 150 �l
Color reagent I 75 �l 75 �l 75 �l
Color reagent II 75 �l 75 �l 75 �l
mix, incubate for 5 min at +15 to
Pipet 100 µl antibody to all tubes (except optional NSB tube).
Test tube rack for 12 x 75 mm test tubes Optional: Add 100 µl assay buffer to NSB tubes.
Procedure
1. Label 12 x 75 mm