each 04 693 132 001
Aprotinin custom fill 10 236 632 103
Bestatin 10 mg 10 874 515 001
Pefabloc® SC PLUS Set I, 100 mg Pefabloc® SC; 5 ml PSC-Protector solution 11 873 601 001
Set II, 1 g Pefabloc® SC;
., 165, 76-85 (1988).
4. Knight, D. E., Botulinum toxin types A, B and D
inhibit catecholamine secretion from bovine
adrenal medullary cells. FEBS Lett., 207(2), 222-
226 (1986).
5. Argoff, C.
humidified incubator with 5% CO2.
Subculturing the Cells
1. hERG1a/1b Inducible HEK293 cells should be passaged at ~ 80-85% confluency. Do not allow the cells to
grow over 85% confluency.
2. Carefully
Pancreatic phospholipase A2 is very stable. The
enzyme is not denatured by 8 M urea, 5 M guanidine
HCl, 10% TCA, or hot (85 °C) 2% SDS.
References
1. De Haas, G.H., et al., Studies on phospholipase A
Pancreatic phospholipase A2 is very stable. The
enzyme is not denatured by 8 M urea, 5 M guanidine
HCl, 10% TCA, or hot (85 °C) 2% SDS.
References
1. De Haas, G.H., et al., Studies on phospholipase A
.25 mM DTT, 0.1 mM PMSF, and 25% glycerol.
Molecular mass: ∼85 kDa
Purity: 70–95% (SDS-PAGE, see Figure 1)
Specific Activity: 103–139 nmole/min/mg (see Figure 2)
Precautions and Disclaimer
determine optimal working dilutions by titration.
References
1. Blanco, F.J. et al., Circ. Res., 103, 1383-1392
(2008).
2. Velasco, S. et al., J. Cell Sci., 121, 913-919 (2008).
3. Bernabeu, C.
volume of 10 �l by incubation for 16 h at 4°C in 66
mM Tris-HCl, 5 mM MgCl2, 5 mM dithiothreitol, 1 mM
ATP, pH 7.5 (at 20°C) resulting in >85 % recovery of
1 µg �DNA fragments. Subsequent re-cutting
of 10 �l by incu-
bation for 16 h at 25° C in 66 mM Tris-HCl, 5 mM
MgCl2, 5 mM Dithiothreitol, 1 mM ATP, pH 7.5 (at 20° C)
resulting in >85 % recovery of 1 µg �DNA × EcoR
Alzheimer’s disease: Erk1 and Erk2
responses to the inflammatory signal bradykinin.
PNAS, 103(35), 13203-7 (2006).
FLAG is a registered trademark and SILu is a
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