until required.
• Wash the residues with 3 × 15 ml ethanol 78 %, then rewash with 2 × 10 ml ethanol 95 % and
3 × 10 ml acetone.
• Dry overnight at 105 °C.
• Cool
until required.
• Wash the residues with 3 × 15 ml ethanol 78%, then rewash with 2 × 10 ml ethanol 95% and
3 × 10 ml acetone.
• Dry overnight at 105 °C.
• Cool and weigh
106 cells/mL with > 95% viability.
• Passage from the GMEM medium to Cellvento™ BHK-200 medium
+ 10% FBS. Subculture at a concentration of 6 × 105 cells/mL.
• Perform minimum 3 passages (100 mL in
3mal mit 15 ml Ethanol 78 % waschen, dann 2mal mit je 10 ml Ethanol 95 % und
3mal mit je 10 ml Aceton nachwaschen.
• Über Nacht bei 105 °C trocknen.
• Abkühlen und auf 0,1 mg genau auswiegen.
•
106 cells/mL with > 95% viability.
• Passage from the GMEM medium to Cellvento®
BHK-200 medium + 10% FBS. Subculture at a
concentration of 6 × 105 cells/mL.
• Perform minimum 3 passages (100 mL in
résidus à 3 reprises avec 15 ml d’éthanol 78 %, puis relaver à 2 reprises avec chaque
fois 10 ml d’éthanol 95 % et à 3 reprises avec chaque fois 10 ml d’acétone.
• Sécher pendant la nuit à
106 cells/mL with > 95% viability.
• Passage from the GMEM medium to Cellvento®
BHK-200 medium + 10% FBS. Subculture at a
concentration of 6 × 105 cells/mL.
• Perform minimum 3 passages (100 mL in
>107 cells/mL with viabilities greater than 95% and
can be maintained for more than 10 days at these densities.
Cultures seeded at low density (2.5 x 105 cells/mL) over multiple
passages do not exhibit
residuos se lavan 3 veces con sendas cantidades de 15 ml de etanol al 78 %, después
2 veces con 10 ml de etanol al 95 %, y 3 veces con 10 ml de acetona.
• Secar durante
.
5. Continue to subculture the cells in EX-CELLTM CD CHO
every 3 - 4 days for 3 - 4 passages using a seeding density
of 5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks. SAFC
Biosciences recommends
.
5. Continue to subculture the cells in EX-CELLTM CD CHO
every 3 - 4 days for 3 - 4 passages using a seeding density
of 5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks. SAFC
Biosciences recommends
flask should
be used to prevent contamination in case of water
backup.
3. An air oven capable of operating at 105 °C or a
vacuum oven set at 70 °C.
4. Desiccator
5.
flask should
be used to prevent contamination in case of water
backup.
3. An air oven capable of operating at 105 °C or a
vacuum oven set at 70 °C.
4. Desiccator
vacuum flask should
be used to prevent contamination in case of water
backup.
3. An air oven capable of operating at 105 °C or a
vacuum oven set at 70 °C.
4. Desiccator
5. Muffle furnace
blood) >70
Candida albicans 10231 103-105 3 d at 28°C
Recovery rate % (with
and without blood) >70
Aspergillus
brasiliensis,
formely A. niger
16404 Growth good / very
.
5. Continue to subculture the cells in EX-CELLTM CD CHO
every 3 - 4 days for 3 - 4 passages using a seeding density
of 5 x 105 cells/mL in 25 cm2 or 75 cm2 flasks. SAFC
Biosciences recommends