Magnetic Bead Panel 1
Kit Catalog# HCMP1MAG-19K
Control Catalog # HCMP1-6019-1
Analyte QC Level Lot # Expected Range Units
Complement C2 Control 1 HCM1-106 11 – 24 ng/mL
Figure 1). The cells appeared
healthy and there was no clumping of cells even at higher
densities. Cell densities in EX-CELLTM 420 were typically
around 6 x 106 cells/mL (range 3.7 x 106 to
9.9 x 106 cells
Day 3, 2.9 x 106 cells/mL with 98.0% viability on Day
4 (24 hours post-dilution), and a peak of 8.9x106 cells/
mL with 98.2% viability on Day 6 (FigureS7a). The
growth profile for both N-1 and N-stages
Reagent prepared in step 1a, at a
concentration of 105 to 106 cells per 100 µL. Incubate in the dark for 15 minutes at room
temperature (18°-24°C).
5. Collect cells by centrifugation as in step 2. Resuspend
-2 ������� pg
IL-2/106 Jurkat cells. The PHA control culture yielded
106 Jurkat cells. The control with no
addition was 106 Jurkat cells.
References
1. Weiss, A., et al.
TiterHigh Sf supports fast
cell growth rates (doubling times of 20-24 hours), high
cell densities of >20 x 106 cells/mL for Sf21 and >10 x
106 cells/mL for Sf9, while maintaining high cell viability
and high
were prepared
at 1.0 x 106 cells/mL (for
chambers A and B) or at
2.0 x 106 cells/mL (for
chambers C and D). Inlet
well 6 was set to flow at
0.25 psi for 0.1 min (step
1 of
production?
We recommend seeding at 1.2 106 viable
cells/mL 18 to 24 hours before transient
transfection. Targeted cell density on the day of
transfection is 2.4–3.5 106 viable cells/mL and
viability 95%
cells/mL, and infected on day
2 at approximately 1 x 106 cells/mL with wild-type Ad5 at a
multiplicity of infection (MOI) of 5. Samples were collected at
24, 48, 72 and 96 hours and were subjected to 3 rounds
should be taken into account in the evaluation. For
example, if 1 ml of the sample added to 100 ml water reveals, after
incubation, 106 bacteria, the real result is 108 bacteria/ml.
1.00778
Microbiology
1.5 x 104 0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm
1.5 x 104 0.5-1 x 105 1.5 50 0.15
24 well 0.5-1 x 105 2-5 x 105 6 100 0.5
12 well 1-2 x 105 2.5-10 x 105 12 100 1.0
6 well 2-5 x 105 1-2 x 106 30 200 2.0
60 mm