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106-28-5
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关键词:'106-28-5'
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TB314 TriEx Sf9 Cells
using fresh
medium prewarmed to 28°C. Overdilution will cause cell death. In general, maximum densities
attained by shake cultures are 5–6 × 106 cells/ml. However, at these densities the cells stop growing
TB329 Sf9 Insect Cells
Flask Size Cell Number Medium Volume
25-cm2 flask 1.0 × 106 5 ml
75-cm2 flask 3.0 × 106 10 ml
150-cm2 flask 6.0 × 106 30 ml
Passaging Suspension Cultures
Sf9 Insect
Growth Characteristics of the expresSFplus Serum-Free Insect Cell Line in EX-CELL420 Serum-Free Media
triplicate shaker flasks (50 mL
volume per 125 mL flask) at a seeding density of
1.5 x 106 cells/mL. Flasks were incubated at 28 C, on an
orbital shaker at 135 rpm. Cell counts and viability were
monitored using
The Effective Use of Protein Kinase Inhibitors
87 88 76
101 96 28 34 77 28 29 63 104 91 9 92 42 94 111 94 70 22 72 104 101 84
87 110 29 12 12 49 66 28 94 79 20 117 64
The Effective Use of Protein Kinase Inhibitors
87 88 76
101 96 28 34 77 28 29 63 104 91 9 92 42 94 111 94 70 22 72 104 101 84
87 110 29 12 12 49 66 28 94 79 20 117 64
Green Chemistry Brochure
StabilizedCPME Stabilized
0
50
100
150
200
250
0 1 2 3 4 5 6 7 8 9 10 11 12 13 14 15 16 17 18 19 20 21 22 23 24
Data Sheet - SAB4200870
Vos WM., ISME J., 6:
1449–58 (2012).
5. Png CW., et al., Am J Gastroenterol., 105:
2420-28 (2010).
6. Zhang H., et al., Proc Natl Acad Sci USA.,
106: 2365-70 (2009).
7. Santacruz A.
Quality Control Ranges: MILLIPLEX® MAP Human Cytokine/ Chemokine Magnetic Bead Panel
pg/mL
Control 2 447 – 928 pg/mL Control 2 497 – 1032 pg/mL
Flt-3L Control 1 106 – 221 pg/mL
MOUSE ANTI-HUMAN BCL-XL
: Recombinant Bcl-XS.
ISOTYPE: IgG3
CLONE NAME: 7B2.5
APPLICATIONS: Flow cytometry: ≤3μg/106 cells 17,18
Immunoprecipitation 15,16
Immunohistochemistry 17,18
Western blotting: ≤ 0.5 μg/mL
HUMAN INTERFERON ALPHA A
1-800-437-7502
www.millipore.com
IF007/28-FEB-2013/NE
HUMAN INTERFERON ALPHA A
CATALOG NUMBER:
IF007 QUANTITY: XX mL ( 5 x 106 units )
LOT NUMBER:
Product Information Sheet - D0260
3. Henion, W.F. et al., Biochem. Biophys. Acta,
148(1), 106-113 (1967).
4. Swislocki, N.I., Anal. Biochem., 38(1), 260-269
(1970).
5. Schwede, F. et al., Pharmacol. Ther., 87(2-3),
199-226
Anti-CD24, clone SN3 - Data Sheet
APPLICATIONS:
Immunohistochemistry: Frozen tissues
Flow Cytometry: 1:50 – 1:100 per 106 cells in 100 µL volume
Optimal working dilutions must be determined by the end user.
SPECIES
Data Sheet - P6236
Optimal pH range: 6.0–9.0
Purity: ∼90% (SDS-PAGE, with an apparent molecular
mass of 28 kDA)
Specific activity: ≥5 units/mg protein at 37 °C
(At 75 °C, the activity is ∼100 units/mg protein)
Unit
Product Information - 95292
I, 237 (1983).
4 Ion selective electrodes in clinical chemistry. A. Lewenstam, Anal. Proc. 28, 106 (1991).
5 U. Oesch, P. Anker, D. Ammann, W. Simon, in: Ion-Selective Electrodes, Eds. E. Pungor, I. Buzás
Product Information Sheet - FLSAR
released by fewer than 10 or as many as 2 × 105
viable somatic cells (sample containing from 400 to
8 × 106 cells per ml). This compares very favorably with
the sensitivity obtained using a microscope with
Product Information Sheet - FLASC
released by fewer than 10 or as many as 2 × 105
viable somatic cells (sample containing from 400 to
8 × 106 cells per ml). This compares very favorably with
the sensitivity obtained using a microscope with
Bulletin - CHROP
co-repressor
that does not bind DNA directly, e.g., EZH2
qPCR 5-10 x 106
Microarray 50 x 106
(5 ChIPs)
Sequencing 100-200 x 106
(10-20 ChIPs)
Table 1: Guidelines for number of cells required
Product Information Sheet - 11348639001
59:766.
3 Krishan, A. (1975) J. Cell Biol. 68:188.
4 Taylor, I.W. (1980) J. Histochem. Cytochem. 28:1021.
5 Crissman, H.A. and Steinkamp, J.A. (1982) Cytometry 3:84.
6 McCarthy, R.C. and Fetterhoff, T.J.
Product Information Sheet - BK101
Protein (mg/ml) = 4.5
5
2. Determine the number of nmoles of IgG in the
sample (IgG, molecular mass ∼150,000)
nmoles/ml = mg protein/ml × 106
mw of IgG
(106
Product Information Sheet - 62558
Nam, G. S. Cha, Bull.
Korean Chem. Soc. 22, 765 (2001).
5 Ion selective electrodes in clinical chemistry. A. Lewenstam, Anal. Proc. 28, 106 (1991).
6 U. Oesch, P. Anker, D. Ammann, W. Simon, in: Ion-Selective
Data Sheet - SAB4200569
1554-
1569 (2011).
4. Laufman, O., et al., EMBO J., 28, 2006-2017
(2009).
5. Richardson, B.C., et al., Proc. Natl. Acad. Sci.
USA, 106, 13329-13334 (2009).
ST,RC,PHC 02/13-1
Data Sheet - G8032
Biol. Chem.,
255(19), 9490-9493 (1980).
5. Sun, H.Q. et al., J. Biol. Chem., 274(47), 33179-
33182 (1999).
6. Yin, H.L. et al., J. Cell Biol., 106(3), 805-812
(1988).
7. Sun, H.Q. et al.
Data Sheet - SAB4200568
1554-
1569 (2011).
4. Laufman, O., et al., EMBO J., 28, 2006-2017
(2009).
5. Richardson, B.C., et al., Proc. Natl. Acad. Sci.
USA, 106, 13329-13334 (2009).
ST,RC,PHC 02/13-1
Data Sheet - T2190
concentration of
5-15 µg/mL is determined to detect TNF
RI/TNFRSF1A in perfusion fixed frozen sections of
mouse intestine.
Flow Cytometry: a working concentration of
2.5 µg/106 cells is determined
Data Sheet - P5810
Anti-CD3 recognizes the CD3 complex
which is composed of 5 chains designated γ,
δ, ε, ζ and η having a molecular mass distribution of 16,
20, and 25 - 28 kDa. The CD3 human lymphocyte
surface antigen is
Data Sheet - C7048
lymphocyte activation studies.
5. Immunoprecipitation of the CD3 antigen.
When assayed by flow cytometric analysis, 5 µL of the
monoclonal antibody will stain 1 x 106 cells with a
fluorescence intensity
Data Sheet - R9011
Anti-CD3 recognizes the CD3 complex
which is composed of 5 chains designated γ, δ ε, ξ and
η having a molecular mass distribution of 16, 20, and
25 - 28 kDa. The CD3 human lymphocyte surface
antigen is a
Protocol - 71259
precipitation.
4. Incubate at room temperature 15 min.
5. Add the transfection mixture to the cells.
6. Incubate the cells for 48 h at 28°C, shaking at 150 rpm.
Protocol- 03-0187-00
samples (n = 5 serum samples, 5 plasma samples)
Sample ID Serum Recovery %
Plasma
Recovery %
Sample 1 101 84
Sample 2 106 106
Sample 3 104 82
Sample 4 109
Product Information Sheet - T0625
Killing of schistosomula
by taurine chloramine and taurine bromamine.
Am. J. Trop. Med. Hyg., 37(1), 106-110 (1987).
11. Petritis, K., et al., A comparative study of
commercial liquid chromatographic
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