C (50%): B (50%) A (50%): C (50%)
A (33%): C (33%): B (33%) A (67%): C (17%): B (17%)
A (17%): C (67%): B (17%) A (17%): C (17%): B (67%)
6=119%
3=114%
2=115%
1=100%
A
B
density (g/L) 2, 4, 6 4
Multiplicity of infection (pfu/cell) 1 x 10-4 , 5 x 10-4, 1 x 10-3 5 x 10-4
Time of infection
(days after cell inoculation)
2, 3, 4 4 (or cell
cellular aggregates and achieved average
cell densities of 2.5 x 106 cells/mL in shaker fl asks, with an
approximate doubling time of 33 hours. HEK 293 cells in
EX-CELL 293 were also infected with wild-type
0.5 x 106 7.5 x 106 15 x 106 30 x 106
Total number of
cells**
Number of trials per total cells (mL) Including Cell
expansion***
(mL)
30 x 106 60 4 2 1 60 210
Carbohydrate Research, 65, 23-33
(1978).
2. Iwasaki, Y. et al., Purification and characterization
of beta-mannosidase from human placenta.
J. Biochem. (Tokyo), 106(2), 331-335 (1989).
3. Frei
determined by dye exclusion (e.g., trypan blue).
For each sample, add 100 µl or 1 x 106 cells per
tube.
2. Add 10 µl of conjugate to tube(s) containing cells to
be stained. Vortex tube gently.
concentration to 2.5 x 106 cells/ml.
II. Cultured Cells
1. Place tissue culture suspension into a 17 x 100 mm tube allowing enough suspension to
provide 2.5 x 106 cells/sample.
2. Centrifuge for 5