containing 0.46 M
NaCl, 2% PVP, and 41 mM sucrose.
Reconstituted protein solution may be stored up to
2 months at –20 °C.
Storage/Stability
Hemocyanin is stable as a dry solid at 2–8 °C for at
least
APPLICATIONS:
Immunohistochemistry: Frozen tissues
Flow Cytometry: 1:50 – 1:100 per 106 cells in 100 µL volume
Optimal working dilutions must be determined by the end user.
SPECIES
the fusion protein is 41 kDa.
Reagent
c-Jun (1-169)-GST fusion protein is supplied as a
solution in Tris buffered saline (TBS), pH 8.0,
containing 25 mM glutathione, 10 mM 2-
mercaptoethanol and 50%
Store at Room Temperature
CAS RN 300815-41-2 (free base)
Synonym: 1′-[2-[4-(Trifluoromethyl)phenyl]ethyl]-
spiro[4H-3,1-benzoxazine-4,4′-piperidin]-2(1H)-one
hydrochloride
NH
O
N
of Histotechnology. Edited by DC Sheehan and BB Hrapchak,
2nded. Mosby, St. Louis, MO, 1980, p 106
2014 Sigma-Aldrich Co. LLC. All rights reserved. SIGMA-ALDRICH is a trademark of
Sigma-Aldrich
, H. et al., Genes Dev., 12, 2735-2747 (1998).
10. Hsu, D.S. et al., Proc. Natl. Acad. Sci. USA, 106,
5312-5317 (2009).
VS,GG,TD,KAA,PHC,MAM 05/19-1
2019 Sigma-Aldrich Co. LLC. All rights
170 (1989).
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and Flk-1 (vascular
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2. Marotta, F. et al., Chemopreventive effect of a
probiotic
slip.
References
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30 minutes at room temperature.
9. Repeat steps 2 and 3.
10. Resuspend cells in initial volume of diluent. For
each sample, add 100 µl or 1 x 106 cells per tube.
11. Add 10 µl of
metalloproteinases-1 (TIMP-1)
transcription. Biochem. Biophys. Acta, 1171, 41-55
(1992).
7. Gonzalez, E., et al., HIV-1 Tat inhibits IL-2 gene
transcription through qualitative and quantitative
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