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106 7426-44-1
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Growth Characteristics of the expresSFplus Serum-Free Insect Cell Line in EX-CELL420 Serum-Free Media
Figure 1). The cells appeared
healthy and there was no clumping of cells even at higher
densities. Cell densities in EX-CELLTM 420 were typically
around 6 x 106 cells/mL (range 3.7 x 106 to
9.9 x 106 cells
Quality Control Ranges: Mouse Acute Phase Magnetic Bead Panel 2
2.7 – 5.6 ng/mL
Control II MAP2-206 15 – 44 ng/mL
SAP Control I MAP2-106 31 – 65 ng/mL
Control II MAP2-206 193 – 401 ng/mL
Data sheet
IST-106-078LR** LabRoll™ 1 Roll 610M x 78 mm
IST-106-078SR** Sterile LabRoll™ 1 Roll 610M x 78 mm
IST-106-115SR*** Sterile LabRoll™ 1 Roll 500M x 115 mm
IST-106-115LR*** VII LabRoll™ 1 Roll
MILLIPLEX®MAP Human Myokine Panel is an optimized quantitative immunoassay that simultaneously measures 15 novel muscle-secreted factors
Irisin FSTL1 OSM IL-6 FGF21 Musclin
1:4 dil 111 85 58 105 100 97 95 74 92 106 73 106 77 91 109
1:8 dil 123 84 48 108 98 91 94 63 87 105 70 110 66
Product Information Sheet - 14591C
2.9 x 106 cells/mL
and the average doubling time was ~ 24.1 hours. The average
density in αMEM + 10% FBS was about 1.4 x 106 cells/mL
and the average doubling time was approximately 35 hours
(n=1 flask
EX-CELL NS0- An Animal-Component Free
Average Doubling
Time (Hours)
2.2 x 106
1.2 x 106
2.9 x 106
1.9 x 106
95.5
84.5
92.0
92.6
16.1
20.0
18.6
22.2
www.safcbiosciences.com
Figure 1
SC-71 Cell Line
0.00E+00
Discover a Better Cell Culture Insert
Cat. No.
0.4
100 x 106 Translucent PTHT06H48 PTHT12H48 PTHT24H48
4 x 106 Clear PCHT06H48 PCHT12H48 PCHT24H48
1.0
22 x 106 Translucent PLRP06H48 PLRP12H48 PLRP24H48
2 x 106 Clear PTRP06H48 PTRP12H48
EX-CELL 302 Serum-Free Medium for CHO Cells
3. Allow the cultures to achieve a minimum density of
1 x 106 cells/mL before subculturing.
4. Subculture into fresh EX-CELLTM 302 as in Step 1 using
normal seed densities.
Culture Techniques
Once
EX-CELL 302 Serum-Free Medium for CHO Cells
3. Allow the cultures to achieve a minimum density of
1 x 106 cells/mL before subculturing.
4. Subculture into fresh EX-CELLTM 302 as in Step 1 using
normal seed densities.
Culture Techniques
Once
Envirocheck®Dip Slides
Density Charts
Bacteria and Yeasts
103 104 105 106 10710
3 104 105 106 107
103 104 105 106 107103 104 105 106 107103 104 105 106 107103 104 105 106 107
CFU/ml, for liquids
Molds
102
Combat Microbes with ProClin Biocidal Preservatives
Broth Agar 3.38 x 106 4.2 x 106 3.30 x 106 6.5 x 106 a
Potato Dextrose Agar 1.93 x 106 2.1 x 106 3.56 x 106 1.31 x 107 a
Sodium Azide
Tryptic Soy Broth Agar
B6-WHITE™ MURINE ES CELL LINE
cells:
• 1 x 25 cm2 flask
• 1 x 75 cm2 flask
• 3 x 10 cm dishes
Product Information Sheet - 14591C
shaker
flasks?
The average cell density achieved during a 3-day passage is
approximately 2.9 x 106 ± 1 x 106 cells/mL in shaker flasks.
What is the average doubling time of HeLa cells
in EX-CELLTM HeLa
Human Oligodendrocyte Differentiation Kit
Neural Progenitor Kit
Includes: 1 x 106 cells + 500 mLs
Maintenance Medium and 50 mL Freezing
Medium
SCC009
ReNcell VM Human Neural Progenitor Kit
Includes: 1 x 106 cells + 500 mLs
Maintenance Medium
Suspension MDCK Cell Culture in EX-CELL MDCK Serum-Free Medium
free-USA 1 800-255-6032
Fax +1 913-469-5584
E-mail info-na@sial.com
Europe
SAFC Biosciences
Smeaton Road, West Portway
Andover, Hampshire SP10 3LF
UNITED KINGDOM
Phone +44 (0)1264-333311
Fax +44 (0)1264
Data Sheet -103686BC
O157) ≤100 ≥1.0*106 Tryptic soy agar
Escherichia coli O26:H11 ≤100 ≥1.0*106 Tryptic soy agar
Escherichia coli O103:H2 ≤100 ≥1.0*106 Tryptic soy agar
Escherichia coli O111:H8 ≤100 ≥1.0*106 Tryptic soy agar
Datasheet - mEHEC Broth
O157) ≤100 ≥1.0*106 Tryptic soy agar
Escherichia coli O26:H11 ≤100 ≥1.0*106 Tryptic soy agar
Escherichia coli O103:H2 ≤100 ≥1.0*106 Tryptic soy agar
Escherichia coli O111:H8 ≤100 ≥1.0*106 Tryptic soy agar
ChemiScreen™ CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT TSH RECEPTOR, GLYCOPROTEIN HORMONE RECEPTOR FAMILY
200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-10 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense 1
mL aliquots into cryopreservation vials.
FAQs- EX-CELLNS0 and EX-CELLSp2/0 Serum-Free Media
free-AUS 1 800-200-404
Fax +61 (0)3-9315-1656
E-mail info-ap@sial.com
EX-CELLTM NS0
Minimum Seeding Density 2 x 105 cells/mL
Passage frequency 3 - 4 days or until
densities reach 1-2 x 106
cells
ChemiScreen™ Calcium-Optimized Stable Cell Line Human Recombinant GPR109A Receptor
200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in 1321N1 Freezing Media (cell densities of
2-10 x 106 are also acceptable if necessary). Dispense 1 mL aliquots into
cryopreservation vials.
ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human Recombinant EP2 Prostanoid Receptor
at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1 mL aliquots into cryopreservation
vials. Freeze
ChemiScreen™ Calcium-Optimized Stable Cell Line Human Recombinant sst<sub>2 </sub>Receptor
200 x g for 5 min. Resuspend cells at 5 x 106 cells/mL in Chem-1 Freezing
Media (cell densities of 2-10 x 106 are also acceptable if necessary). Dispense 1
mL aliquots into cryopreservation vials.
EX-CELL 405 Serum-Free Medium for Insect Cells
Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension to sterile
cryovials.
6. Place the vials at -20
ChemiScreen™ Human Recombinant XCR1 Chemokine Receptor Calcium-Optimized Stable Cell Line
Phone: +1(800) 437-7500 • Fax: +1 (951) 676-9209 • Europe +44 (0) 23 8026 2233
Australia +61 3 9839 2000
www.millipore.com
12/12/12/DJ/HTS053C/KS
PRESENTATION:
Cells are frozen at 2 x 106 cells
ChemiScreenTM GLOW AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x
106 are also acceptable if necessary). Dispense 1 mL aliquots into cryopreservation
vials. Freeze
ChemiScreen™ FLASH AEQUORIN CALCIUM-OPTIMIZED STABLE CELL LINE HUMAN RECOMBINANT M1 MUSCARINIC ACETYLCHOLINE RECEPTOR
at 200 x g for
5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10 x
106 are also acceptable if necessary). Dispense 1 mL aliquots into cryopreservation
vials. Freeze
EX-CELL 610-HSF Serum-Free Medium for Hybridoma Cells
Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension to sterile
cryovials.
6. Place the vials at -20
EX-CELL 420 Serum-Free Medium for Insect Cells
Remove the
supernatant.
4. Resuspend the cells in the freezing medium at 5 x 106 to
1 x 107 cells/mL.
5. Rapidly transfer 1 - 2 mL of this suspension to sterile
cryovials.
6. Place the vials at -20
EX-CELL 420 Frequently Asked Questions
minutes.
Remove the supernatant. Resuspend the cells in the freezing
medium at 5 x 106 to 1 x 107 cells/mL. Rapidly transfer
1 - 2 mL of this suspension to sterile cryovials. Place the vials
at -20 C for 3
ChemiScreen™ Flash Aequorin Calcium-Optimized Stable Cell Line Human Recombinant EP3 Prostanoid Receptor
at 200 x g
for 5 min. Resuspend cells at 5 x 106 cells/mL in Freezing Media (cell densities of 2-10
x 106 are also acceptable if necessary). Dispense 1 mL aliquots into cryopreservation
vials. Freeze
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