MILLIPLEX® MAP Human Cancer Biomarker Magnetic Bead Panel 4
Kit Catalog# HCCB4MAG-58K
Control Catalog# HCC4-6058-4, Lot# HCC4-106 and HCC4-206
Note: The Quality Control Ranges are generated
clarification of a CHO centrate feed stream (mAb02, 173 NTU, cell
culture harvest details: 16.9 x 106 TC/mL, 93% viable). Filter resistance profiles are shown in the top panel and filter turbidity breakthrough
degree) of 4-nitrophenol at pH 5.5, and the
millimolar absorbance coefficient of 4-nitro-
phenoxide in the presence of 0.5% DEn-CD.
References
1. Borooah, J. et al., Biochem. J., 78(1), 106-110
Neurotrophins and
the Neural Crest, Sieber-Blum, M., ed., CRC (Boca
Raton, FL: 1999), pp. 59-93.
4. Callard, R., and Gearing, A., The Cytokine Facts
Book, Academic Press (New York, N.Y.: 1994).
from BALB/c mice immunized with a
synthetic phosphorylated peptide corresponding to
amino acids 93-106 (pTyr99) of human MDMX (Gene ID:
4194). The isotype is determined using a double
diffusion immunoassay
with PBS, being careful not
to dislodge any of the cells.
3. Discard the PBS.
4. Add cell lysis buffer (106 – 107 cells/mL).
For cells in suspension
1.
approximately 1-3 x 106 cells/mL, with culture viabilities in
excess of 93%, when subcultured every 3 - 4 days (Figure 2).
The growth curve (Figure 3) indicates that maximal cell
densities nearing 5 x 106 cell/mL
et al., Proc. Nat. Acad. Sci., USA, 76,
4061 (1979).
4. Biddison, W., et al., Immunol., 131, 152 (1983).
5. Maddon, P., et al., Cell, 42, 93 (1985).
6. Dalgleish, A., et al., Nature, 312, 763 (1986)
c-myc expression in
rat colon carcinogenesis induced by
azoxymethane. J. Gastroenterol., 37, 186-93
(2002).
SS,VNC,MAM 04/19-1
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