EDTA
Concentration: 700 µg/ml or 14.0 A260 units/ml
Ratio: A260/A280: 1.8
M.W.: 31.5 x 106 Daltons
BASE PAIRS: 48,502
Unique Sites: Apa I, Nae I, Nar I Nhe I, PaeR7
EDTA
Concentration: 495 µg/ml or 9.9 A260 units/ml
Ratio: A260/A280: 1.9
M.W.: 31.5 x 106 Daltons
BASE PAIRS: 48,502
Unique Sites: Apa I, Nae I, Nar I Nhe I, PaeR7 I, SnaB
EDTA
Concentration: 445 µg/ml or 8.9 A260 units/ml
Ratio: A260/A280: 1.9
M.W.: 31.5 x 106 Daltons
BASE PAIRS: 48,502
Unique Sites: Apa I, Nae I, Nar I Nhe I, PaeR7 I, SnaB
EDTA
Concentration: 556 µg/ml or 11.1 A260 units/ml
Ratio: A260/A280: 1.9
M.W.: 31.5 x 106 Daltons
BASE PAIRS: 48,502
Unique Sites: Apa I, Nae I, Nar I Nhe I, PaeR7 I, SnaB
380
400
1.0
0.9
0.8
0.7
0.6
0.5
0.4
0.3
0.2
0.1
0
A
b
s
o
r
b
a
n
c
e
7/99
Sigma brand products are sold through Sigma-Aldrich, Inc.
Sigma-Aldrich, Inc.
studied by
kinetochore staining in the cytokinesis-blocked
micronucleus assay. Mutat. Res., 490(2), 99-106
(2001).
5. Ermolli, M., et al., Nickel, cobalt and chromium-
induced cytotoxicity and intracellular
flask unless otherwise indicated. The day prior to
transfection, the cells were passaged at 0.5 x 106 cells/mL
and diluted to a concentration of 1x106 cells/mL the day
of transfection.
All
Performance
When assayed by flow cytometric analysis, using 10 µl
of the conjugate to stain 1 x 106 cells, a fluorescence
intensity and percent positive is observed similar to that
obtained with saturating