Στροφέας περιστροφικού κάδου
Στροφέας σταθερής γωνίας
PR05779w Rev 10/21 35 of 107
Μεγιστοποίηση της ανάκτησης δείγματος
Χαμηλή ανάκτηση δείγματος στο συμπύκνωμα μπορεί να οφείλεται
). Clarified lentivirus (~ 3.9 x 107 Ivp) was purified using the Fast-Trap purification device, and efficient
recovery observed (avg. 72%). The eluted virus (~ 2 x 107 Ivp) was concentrated/buffer exchanged
Repeat the Wash Step once again without
counting the cells.
7. Add 1-2.5 mL of the prepared 1× Extraction
Buffer A per 2-5 × 107 cells. Incubate on ice for
10-15 minutes.
8. Homogenize
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ER/MCT/PHC 10/04
Sigma brand products are sold through Sigma-Aldrich
Pseudomonas fluorescens and
Pseudomonas putida in the clinical laboratory, Appl. Microbiol., 25, 107 (1973)
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Extracts –
A. Cell Extract Preparation
The procedure is for cell extract preparations from
1–6 × 107 cells (in suspension or adherent cells) in a
150 cm2 tissue culture flask.
1. Collect the cells from
substrate
specific
+20 to
+35°C
15 – 60 min
Step 8 Evaluation
light microscope
Between
steps
Washing wash buffer PBS/TBS 7.2 – 8.0 +20 to
+35°C
3 × 5 min
Step 7 Substrate a) Fast