molecular weights of 59 kDa and 104 kDa.1
Botulinum toxins A, B, and D have been reported to
inhibit catecholamine secretions.4 Botulinum toxin B
has been used to study neuropathic pain,5 spasticity,6
extraction of
106 – 107 cells, or of 5-20 mg tissue,
prepare 1 mL of cell lysis buffer, such
as follows:
Component Volume
5× Buffer (Tris-EDTA) 200 µL
5× NaCl
-FITC
Clone WM-59
produced in mouse, purified immunoglobulin
Catalog Number F8402
Product Description
Monoclonal Anti-CD31 (PECAM-1) (mouse IgG1
isotype) is derived from the WM-59 hybridoma
produced
599 (2000).
4. O’Carroll, D., et al., Mol. Cell. Biol., 20, 9423-9433
(2000).
5. Peters, A.H.F.M., et al., Cell, 107, 323-327 (2001).
ImmunoType is a trademark of Sigma-Aldrich Biotechnology.
suspension to 1 x 107 cells/ml in
Diluent. Cells should be >90% viable as
determined by dye exclusion (trypan blue). For
each sample, add 100 µl or 1 x 106 cells per
tube.
2. Add 5
vaccine delivery as a physical immune
enhancer". University of Queensland, Ph.D.
dissertation, p. 107 (2014).
12. Hemphill, Daniel, "Development of an scAAVIGF-I
Gene Therapeutic Vector for the Enhancement
of 2× Dye Solution (step 5) and immediately mix by
pipetting. Do not mix by “racking” or vortexing. Final
concentrations after mixing the indicated volumes will be
1 × 107 cells/mL and 2 ×10–6 M of
of 2× Dye Solution (step 5) and immediately mix by
pipetting. Do not mix by “racking” or vortexing. Final
concentrations after mixing the indicated volumes will be
1 × 107 cells/mL and 2 ×10–6 M of
et al., Proc. Nat. Acad. Sci. USA,
107(11): 4908-4913 (2010).
9. Valdez-Sinon, A.N. et al., iScience, 23(5):
101132 (2020).
10. Huang, D. et al., Biochemistry, 59(16):
1559-1564 (2020).
Notice
Flow cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent. Cells should be >90% viable as
determined by dye exclusion (e.g., trypan blue
cytometer.
Procedure
1. a. Use 100 µl of whole blood or
b. Adjust cell suspension to 1 x 107 cells/ml in
diluent. Cells should be >90% viable as
determined by dye exclusion (e.g., trypan
step 4) to
1 mL of 2× Dye Solution (step 5) and immediately mix
the sample by pipetting. Final concentrations after mixing
the indicated volumes will be 1 × 107 cells/mL and
2 ×10–6 M PKH67.
Note 1
step 4) to
1 mL of 2× Dye Solution (step 5) and immediately mix
the sample by pipetting. Final concentrations after mixing
the indicated volumes will be 1 × 107 cells/mL and
2 ×10–6 M PKH67.
Note 1