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Soledad Sagastume et al.
The Journal of eukaryotic microbiology, 61(1), 42-50 (2013-10-10)
To date, few organisms have been shown to possess variable ribosomal RNA, otherwise considered a classic example of uniformity by concerted evolution. The polymorphism for the 16S rRNA in Nosema ceranae analysed here is striking as Microsporidia are intracellular parasites
Nayuta Yakushiji-Kaminatsui et al.
Methods in molecular biology (Clifton, N.J.), 2529, 207-228 (2022-06-23)
Among the most important histone methyltransferases for metazoan development are EZH1/2 and their homologs, which methylate histone H3 lysine 27 and act as part of a highly conserved set of chromatin regulators called Polycomb Group (PcG) proteins. Reaching a precise
David Guérit et al.
Methods in molecular biology (Clifton, N.J.), 1672, 387-402 (2017-10-19)
Telomerase, the enzyme that elongates telomeres in most eukaryotes, is a ribonucleoprotein complex composed of a reverse transcriptase catalytic subunit (TERT in human, Est2 in the budding yeast S. cerevisiae), regulatory factors and a noncoding RNA called hTERC (in human)
Xiao Shu et al.
STAR protocols, 3(1), 101096-101096 (2022-01-22)
We describe here a metabolic labeling protocol for detecting cellular transcriptome-wide mRNA N 6 -methyladenosine (m6A) at base resolution. By feeding cells an analog of methionine, potential mRNA m6A forming sites are replaced with the N 6 -allyladenosine (a6A). A
Shravan K Chintala et al.
International journal of molecular sciences, 24(2) (2023-01-22)
There is a significant unmet need for therapeutics to treat ocular surface barrier damage, also called epitheliopathy, due to dry eye and related diseases. We recently reported that the natural tear glycoprotein CLU (clusterin), a molecular chaperone and matrix metalloproteinase
Wejdene Gongi et al.
Journal of polymers and the environment, 30(11), 4738-4748 (2022-08-30)
Cyanobacterial extracellular polymeric substances "EPS" have attracted intensive concern in biomedicine and food. Nevertheless, the use of those polymers as a sensor coating material has not yet been investigated mainly for microplastic detection. This study focuses on the application of
Gal Haimovich et al.
Bio-protocol, 8(21), e3070-e3070 (2018-11-05)
Transcription and RNA decay play critical roles in the process of gene expression and the ability to accurately measure cellular mRNA levels is essential for understanding this regulation. Here, we describe a single-molecule fluorescent in situ hybridization (smFISH) method (as
Wejdene Gongi et al.
Marine drugs, 20(4) (2022-04-22)
Cyanobacteria, particularly thermophilic strains, represent an important potential source of EPSs, harboring structural complexity that predicts diverse and specific bioactive potential. The thermophilic cyanobacteria Gloeocapsa gelatinosa, isolated from a natural hot source in Ain Echfa (Tunisia), was cultivated in a
Ye Yuan et al.
Frontiers in physiology, 13, 1051544-1051544 (2022-11-29)
Circadian clocks are ∼24-h timekeepers that control rhythms in almost all aspects of our behavior and physiology. While it is well known that subcellular localization of core clock proteins plays a critical role in circadian regulation, very little is known
Jennifer L Modesto et al.
Nature communications, 14(1), 275-275 (2023-01-18)
Glycans facilitate critical biological functions and control the mammalian gut microbiota composition by supplying differentially accessible nutrients to distinct microbial subsets. Therefore, identifying unique glycan substrates that support defined microbial populations could inform therapeutic avenues to treat diseases via modulation
Florian Clemente et al.
Cell, 184(10), 2565-2586 (2021-05-01)
The Cycladic, the Minoan, and the Helladic (Mycenaean) cultures define the Bronze Age (BA) of Greece. Urbanism, complex social structures, craft and agricultural specialization, and the earliest forms of writing characterize this iconic period. We sequenced six Early to Middle
Lelde Kalnina et al.
APMIS : acta pathologica, microbiologica, et immunologica Scandinavica, 129(7), 393-400 (2021-03-18)
The SARS-CoV-2 pandemic has created an urgent need for diagnostic tests to detect viral RNA. Commercial RNA extraction kits are often expensive, in limited supply, and do not always fully inactivate the virus. Together, this calls for the development of
Agnès Dubois et al.
Development (Cambridge, England), 149(17) (2022-08-18)
Mouse embryonic stem cells have an inherent propensity to explore gene regulatory states associated with either self-renewal or differentiation. This property depends on ERK, which downregulates pluripotency genes such as Nanog. Here, we aimed at identifying repressive histone modifications that
Janske van de Crommenacker et al.
The Journal of experimental biology, 213(Pt 20), 3527-3535 (2010-10-05)
One route to gain insight into the causes and consequences of ecological differentiation is to understand the underlying physiological mechanisms. We explored the relationships between immunological and oxidative status and investigated how birds cope physiologically with the effects of immune-derived
Topaz Altman et al.
Nature communications, 12(1), 6914-6914 (2021-11-27)
Mislocalization of the predominantly nuclear RNA/DNA binding protein, TDP-43, occurs in motor neurons of ~95% of amyotrophic lateral sclerosis (ALS) patients, but the contribution of axonal TDP-43 to this neurodegenerative disease is unclear. Here, we show TDP-43 accumulation in intra-muscular
Xavier Fernandez-Cassi et al.
Water research, 200, 117252-117252 (2021-05-29)
Wastewater-based epidemiology (WBE) has been shown to coincide with, or anticipate, confirmed COVID-19 case numbers. During periods with high test positivity rates, however, case numbers may be underreported, whereas wastewater does not suffer from this limitation. Here we investigated how
Olga A Kovalenko et al.
Current protocols in human genetics, Chapter 19, Unit 19-Unit 19 (2009-07-08)
This unit describes the gene-specific quantitative PCR-based (QPCR) assay, which is used to measure DNA integrity of both nuclear and mitochondrial genomes based on amplification of long DNA targets. QPCR can be used to quantify the formation of DNA damage
Valeria De Pasquale et al.
Journal of medicinal chemistry, 66(3), 1790-1808 (2023-01-26)
Sanfilippo syndrome comprises a group of four genetic diseases due to the lack or decreased activity of enzymes involved in heparan sulfate (HS) catabolism. HS accumulation in lysosomes and other cellular compartments results in tissue and organ dysfunctions, leading to
Qian-Qian Han et al.
Redox biology, 58, 102543-102543 (2022-11-28)
Vitamin C, a key antioxidant in the central nervous system, cycles between ascorbic acid and dehydroascorbic acid under pathophysiological conditions. Clinical evidence supports that the absence of vitamin C may be linked to depressive symptoms, but much less is known
Guiping Kong et al.
STAR protocols, 3(1), 101166-101166 (2022-05-11)
The study of neuronal signaling ex vivo requires the identification of the proteins that are represented within the neuronal axoplasm. Here, we describe a detailed protocol to isolate the axoplasm of peripheral and central axonal branches of sciatic dorsal root
Kristopher D Barbee et al.
Macromolecular bioscience, 11(5), 607-617 (2011-02-10)
A method for fabricating DNA polymer brush arrays using photolithography and plasma etching followed by solid-phase enzymatic DNA amplification is reported. After attaching oligonucleotide primers to the surface of a glass coverslip, a thin layer of photoresist is spin-coated on
P Bourassa et al.
The journal of physical chemistry. B, 114(9), 3348-3354 (2010-02-13)
We report the complexation of bovine serum albumin (BSA) with resveratrol, genistein, and curcumin, at physiological conditions, using constant protein concentration and various polyphenol contents. FTIR, CD, and fluorescence spectroscopic methods were used to analyze the ligand binding mode, the
Guido Alberto Gnecchi-Ruscone et al.
Cell, 185(8), 1402-1413 (2022-04-03)
The Avars settled the Carpathian Basin in 567/68 CE, establishing an empire lasting over 200 years. Who they were and where they came from is highly debated. Contemporaries have disagreed about whether they were, as they claimed, the direct successors
Guillaume Méric et al.
Environmental microbiology, 15(2), 487-501 (2012-09-01)
Plants are increasingly considered as secondary reservoirs for commensal and pathogenic Escherichia coli strains, but the ecological and functional factors involved in this association are not clear. To address this question, we undertook a comparative approach combining phenotypic and phylogenetic
Oriol Iborra-Egea et al.
Methods in molecular biology (Clifton, N.J.), 2454, 61-81 (2021-12-01)
Stem cell therapy has an unparalleled potential to treat blood cancers, cardiovascular diseases and neurodegenerative conditions, among others. However, stem cell therapeutics must overcome multiple requirements before reaching clinical trials, including large animal safety and efficacy studies. In cardiovascular diseases
A Robust Protocol to Isolate Outer Membrane Vesicles from Nontypeable Haemophilus influenzae.
Libardo, et al.
Methods and Protocols, 6 (2023)
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