water bath.
6. Prepare a suspension of 5 x 108 cells/ml of
antibody sensitized sheep erythrocytes, EA7S,
(Product No. E9383) in ice cold GVB2+.
7. Pipette 400 µl of the antibody sensitized sheep
Prepare a suspension of 1.5 x 108 cells/ml of
antibody sensitized sheep erythrocytes (Product
No. E9383, EA7S) in GVB2+.
6. Prepare reaction tubes according to Table 1.
7. Incubate all tubes in a 37EC
cultured cells (~3 × 108 cells)
• Perform the whole procedure at 2-8 °C.
• All the solutions and equipment should be
pre-cooled before use.
• Homogenize the samples using a 7
β-mercaptoethanol to 14ml of denaturating solution per
108 cells. This solution can be stored one month at room temperature.
2. Add 10ml of solution D per 108 cells. Mix thoroughly and allow to sit 1 min at
C1q with GVB2+.
6. Prepare a suspension of 1.5 x 108 cells/ml of
antibody sensitized sheep erythrocytes (Product
No. E9383, EA7S) in GVB2+.
7. Prepare reaction tubes on ice according to Table 1.
From Cell Cultures (~2 × 108 cells)
Perform the procedure at 4 °C. All solutions and
equipment should be cooled before use. Homogenize
the samples using a 7 mL Dounce glass tissue grinder
co-cultures: effects of a statin, an
ACE-inhibitor and a low-molecular-weight heparin.
Thromb. Res., 108(1), 77-84 (2002).
8. Volpini, R. A., et al., Effect of enalapril and
losartan on the events that
00120354, Rev. A, 11/14
Specifications
Insert Dimensions 24-well 12-well 6-well
Height 16 mm 16 mm 16 mm
Outer diameter 9 mm 15 mm 27 mm
Inner diameter 6.5 mm 12 mm 24 mm
Membrane area (
, et al., Cell, 87, 1327-1338 (1996).
7. McHugh, T. J., et al., Cell, 87, 1339-1349 (1996).
8. Advokat, C. and Pellegrin, A. I., Neurosci.
Biobehav. Rev., 16, 13-24 (1992).
9. Beal, M. F., FASEB
, et al., Cell, 87, 1327-1338 (1996).
7. McHugh, T. J., et al., Cell, 87, 1339-1349 (1996).
8. Advokat, C. and Pellegrin, A. I., Neurosci.
Biobehav. Rev., 16, 13-24 (1992).
9. Beal, M. F., FASEB
Catalog# MHSTCMAG-70K, MHSTCMAG-70KPMX, and MHSTCMAG-70KPXBK
Control Catalog # MHSTC-6070, Lot # HSTC-108 and HSTC-208
MHSTC-6070: HSTC-108.208 (01 February 2024)
EMD MILLIPORE • 1-800-645-5476 • 1
, et al., Cell, 87, 1327-1338 (1996).
7. McHugh, T. J., et al., Cell, 87, 1339-1349 (1996).
8. Advokat, C. and Pellegrin, A. I., Neurosci.
Biobehav. Rev., 16, 13-24 (1992).
9. Beal, M. F., FASEB