solution per
108 cells. This solution can be stored one month at room temperature.
2. Add 10ml of solution D per 108 cells. Mix thoroughly and allow to sit 1 min at room
temperature.
us to trace the manufacturing
history of your column.
11-108-5_PB_Chromolith prep SI:S000002122_Chromolith prep SI 16.11.11 09:56 Seite 3
1.3.1 Installation of the Column
Connect your
charged membrane
108
(breakthrough point
not reached)
>8.2 x 108 >6.04 x 108
Figure 4.
Observed endotoxin
concentrations
Figure 3.
5% mannitol
solutions spiked with
of 108 EU/mL (trial 1
prepare a minimum
of 2 × 108 cells and 10 mL of buffer.
1× OptiPrep™ Dilution Buffer
1. Dilute an aliquot of the OptiPrep™ Dilution Buffer
20× (Component O4889) 20-fold with water.
nmole/min/mg = ∆cpm × (25/20)
SR × E × T
SR = specific radioactivity of the ATP (cpm/nmole ATP)
∆cpm = cpm of the sample – cpm of the blank (step 3)
25 = total reaction volume
20 = spot volume
T
deionized water in the amount given on the label.
2. Add 20 µl of a solution containing 0.3 M CaCl2
and 1 M MgCl2 to each ml of C1q deficient serum.
3. Prepare nine precooled assay tubes labeled "A"
through
serum with 1 ml ice
cold deionized water.
2. Add 20 µl of a solution containing 0.3 M CaCl2 and
1 M MgCl2 to each ml of C1q deficient serum.
3. Dilute an aliquot of the C1q deficient serum with
gelatin
limiting, 1 x 108 cells is a
useful guideline quantity.
• Stimulate or treat, if necessary, adherent mammalian cells at ~80-90% confluence in a 150
mm culture dish containing 20 mL of growth
;23:77.
2 Wieger U & Hilz H. Biochem. Biophys. Res. Commun. (1971);44:513.
3 Hilz, H. et al. Eur. J. Biochem. (1975);56:103–108.
4 Sambrook J et al. Molecular Cloning: A Laboratory Manual, 2nd edition