which causes
50% inhibition of lysis of 2 x 108 antibody sensitized
sheep erythrocytes (Product No. E9383) when
incubated with human complement serum at 37 °C for
15 minutes in a final volume of 1
precooled assay tubes labeled "A"
through "H" and 2 precooled control tubes labeled
"Spontaneous Lysis" and "100% Lysis".
2. Thaw the C2 deficient serum in a 37 °C water bath.
Do not thaw at 4 °C or at room
precooled assay tubes labeled "A"
through "H" and 2 precooled control tubes labeled
"Spontaneous Lysis" and "100% Lysis".
2. Thaw the C5 deficient serum in a 37 °C water bath.
Do not thaw at 4 °C or at room
pre-cooled assay tubes labeled "A"
through "H" and 2 pre-cooled control tubes labeled
"Spontaneous lysis" and "100% lysis".
2. Thaw the C6 deficient serum in a 37 °C water bath.
Do not thaw at 4 °C or at room
precooled assay tubes labeled "A"
through "H" and 2 precooled control tubes labeled
"Spontaneous Lysis" and "100% Lysis".
2. Thaw the C7 deficient serum in a 37 °C water bath.
Do not thaw at 4 °C or at room
precooled assay tubes labeled "A"
through "H" and 2 precooled control tubes labeled
"Spontaneous Lysis" and "100% Lysis".
2. Thaw the C4 deficient serum in a 37 °C water bath.
Do not thaw at 4 °C or at room
precooled assay tubes labeled "A"
through "H" and 2 precooled control tubes labeled
"Spontaneous Lysis" and "100% Lysis".
2. Thaw the C3 deficient serum in a 37 °C water bath.
Do not thaw at 4 °C or at room
precooled assay tubes labeled "A"
through "H" and 2 precooled control tubes labeled
"Spontaneous Lysis" and "100% Lysis".
2. Thaw the C8 deficient serum in a 37 °C
water bath. Do not thaw at 4 °C or at
room
of 1.5 x 108 cells/ml of
antibody sensitized sheep erythrocytes (Product
No. E9383, EA7S) in GVB2+.
7. Prepare reaction tubes on ice according to Table 1.
8. Incubate all tubes in a 37°C water bath
Magnetic Bead Panel 2
Kit Catalog # HSP2MAG-63K
Control Catalog # HSP2-6063-2, Lot# HSP2-108 and HSP2-208
Analyte QC Level Expected Range Units
Granzyme B Control I 18 - 37 pg/mL
min at +37°C.
Isolation of genomic DNA from mammalian tissue (5): The start-
ing material can be 80 mg minced mammalian tissue, 80 mg of tissue
that has been frozen in liquid nitrogen, or 1 × 108 cultured
of Polyguanylic acid
by polynucleotide phosphorylase of Escherichia
coli. Biochim. Biophys. Acta, 108, 125-131 (1965).
RBG,EB,MAM 12/09-1
Sigma brand products are sold through Sigma-Aldrich, Inc.
tightly closed and incubated for 20 to
21 days at 35-37 °C. They are monitored every 2-3 d ays and are subcultured, if a color change occurs.
On days 2-4, 6-8, 13-15, and 19-21 after inoculation the
-protruding ends for 10 minutes at +37°C.
– Incubate DNA with sticky 5′-recessive ends for 30 minutes at +37°C.
Inactivate the rAPid Alkaline Phosphatase for 2 minutes at +75°C.
Use the dephosphorylation
be tested. The liquid media are tightly closed and incubated for 20 to 21 days at 35-37
°C.
On days 2-4, 6-8, 13-15, and 19-21 after inoculation the liquid media (article number 146311)