Enterobacteriaceae were tested within a validation study. No signals
were observed using 2.3 x 108 Enterobacter cloacae cells or 7 x 108 Citrobacter freundii cells per assay,
whereas clear specific signals were detectable
the
USP Safety Test.
Viral Retention Rated for aerosol retention of a total challenge of 108–1010 plaque forming
units of φX–174 virus particles at 50 SCFM per 10‑inch cartridge.
Bacterial
5 minutes to pellet cells.
12. Re-suspend pellet in 10 mL of 1X chilled PBS per 108 cells. Transfer 5 x 107 to 1 x 108 cells
to 15 mL conical tubes and spin at 1,350 x
contains the estimated
amount of 40 µg V5-tagged protein. Incubate for
60 minutes at 37 °C using a rotating platform.
6. Collect the flow-through of unbound protein.
7. Wash with PBS until the absorbance
Nutr., 71(1), 103-108
(2000).
5. Lambert, J.D. et al., Drug Metab. Dispos., 34(12),
2111-2116 (2006).
6. Manach, C. et al., Eur. J. Clin. Nutr., 57(2), 235-242
(2003).
7. Wang, P. et al.
with Step 12, (cloning), the cell surface must be free
of serum.
In a conical tube, mix 1 × 108 spleen cells (in 25 ml) and 2 × 107 myeloma cells (in 25 ml) in serum-free
the invoice or packing slip.
7. Berg T., and Johansson SGO., Int Arch Allergy,
36, 219-32 (1969).
8. Busse W., et al., J Allergy Clin Immunol., 108,
184-90 (2001).
9. Zellweger
, 1 x 25 ml 03 315 959 001
100 ml, 4 x 25 ml 03 315 843 001
Poly(A) x (dT)15 5 U, A260 units 10 108 677 001
Deoxynucleoside
Triphosphate Set
4 x 250 μl, 4 x 25
. Southwick, P. L., et al., Cytometry, 11, 418 (1990).
2. Albiges-Riso, C., et al., J. Cell Sci., 108, 3317
(1995).
3. Herman, I. M., Curr. Opin. Cell Biol., 5, 48 (1993).
4. Vandekerckhove, J.
Concentration (ng/mL)
Standard 1 0.02 0
Standard 2 0.20 20
Standard 3 0.35 40
Standard 4 0.67 75
Standard 5 1.21 150
Standard 6 1.86 300
Standard 7 2.47 600
Standard 8 3.04 1200
3
Product Profile
Sensitivity
Procedure #2
Large (0.1 to 1.0 g tissue or 107-108 mammalian cells) Procedure #3
Other yeast cells Procedure #4
Viral particles Procedure #5
7
Use of RNase
If the
Azacytidine, DNA
methylation, and differentiation. Current Topics in
Microbiology and Immunology, 108, 115-127
(1984).
4. Jones, P.A. at al., Inhibition of DNA methylation by
5-azacytidine
230 V
and 100 V pumps.
P36512_RevE.indd 7 5/20/2009 3:17:08 PM
8 www.millipore.com
Environmental Conditions
Temperature 5 °C (41 °F) to 40 °C (104 °F)
Relative Humidity, Max. 80%
Constant
The affinity constant (Ka) is determined by a Scatchard
plot using this RIA system.
Ka = 1 x 108 − 1 x 109 L/mole.
MG,KAA,PHC 09/07-1
Sigma brand products are sold through Sigma-Aldrich, Inc
Constant
The affinity constant (Ka) is determined by a Scatchard
plot using this RIA system.
Ka = 1 x 108 − 1 x 109 L/mole.
MG,KAA,PHC 09/07-1
Sigma brand products are sold through Sigma-Aldrich, Inc
optimal working dilutions by titration.
References
1. Gimona, M., et al., Cell Motil. Cytoskel., 27, 108-
116 (1994).
2. North, A.J., et al., J. Cell. Sci., 107, 445-455
(1994).
3. North, A.J., et
optimal working dilutions by titration.
References
1. Gimona, M., et al., Cell Motil. Cytoskel., 27, 108-
116 (1994).
2. North, A.J., et al., J. Cell Sci., 107, 445-455 (1994).
3. North, A.J., et al.
of
FAK regulates centrosome-associated
miocrotubules and neuronal migration. Cell Cycle,
3(2), 108-10 (2004).
5. Tomizawa, K. et al., Cophosphorylation of
amphiphysin I and dynamin I by Cdk5 regulates