with Step 12, (cloning), the cell surface must be free
of serum.
In a conical tube, mix 1 × 108 spleen cells (in 25 ml) and 2 × 107 myeloma cells (in 25 ml) in serum-free
activin
receptor cDNA reveals high evolutionary
conservation. Biochem. Biophys. Acta, 1130, 105-
108 (1992).
2. de Winter, J., et al., Activin is produced by rat
Sertoli cells in vitro and can act
Insulin Solution (Catalog Number I9278)
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2–8 °C.
10.8 µL of EGF solution (1
contribution to
antibiotic resistance mechanisms". University of
Warwick, Ph.D. dissertation, p. 108 (2018).
21. Marin, Lina Maria, "Effect of histatin- and
statherin-derived engineered salivary
cAMP-dependent Transcription”.
University of California San Francisco, Ph.D.
dissertation, p. 108 (2019).
Notice
We provide information and advice to our customers
on application technologies
Mobile Phase B: 0.06% Formic acid in methanol
Flow: 0.6 mL/min
Gradient: 60% to 75% B in 8 min; hold at 75% B for 4 min
Injection: 0.5 µL, 25 mg/L
Column Temp.: 45 °C
20 g (wet weight) of bovine liver, or in 10 mL of cell
lysate from CHO cells at a cell density of 108 cells per
mL. CHO cells were grown on DMEM with 10% FCS
(heat-inactivated).
Note: Not
Insulin Solution (Catalog Number I9278)
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2–8 °C.
10.8 µL of EGF solution (1
culture and wash three times with physiological saline (0.85% NaCl)
and adjust to a microbial count of 108 bacteria/ml.
Calibration
Dissolve 100 mg of vitamin B12 (Cat. No. 95190) in 1 litre of distilled
following:
1 liter of DMEM/F12
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2-8 °C.
10.8 µL of EGF solution (1
Insulin Solution (Catalog Number I9278)
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2–8 °C.
10.8 µL of EGF solution (1
StreptaWell plate in concentrations >0.1 to 1%.
• pH 4 to 10
• Elevated temperatures up to +75°C.
Do not heat above +75°C.
Sensitivity
Potential sensitivity-limiting factors for a microplate assay include
Insulin Solution (Catalog Number I9278)
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2–8 °C.
10.8 µL of EGF solution (1
StreptaWell plate in concentrations >0.1 to 1%.
• pH 4 to 10
• Elevated temperatures up to +75°C.
Do not heat above +75°C.
Sensitivity
Potential sensitivity-limiting factors for a microplate assay include
μCi dXTP or 0.5 μg DNA and 100 μCi dXTP.
The standard assay will give a specific activity of 3 × 108 dpm/μg, corresponding to 65% incorporation with different
substrate DNAs, such as pBR322, λDNA, or
Insulin Solution (Catalog Number I9278)
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2–8 °C.
10.8 µL of EGF solution (1
following:
1. 1 liter of DMEM/F12
2. 108 µL of Cholera toxin solution (1 mg/mL),
prepared by dissolving Catalog Number C8052 in
sterile water. Store solution at 2-8 °C.
3. 10.8 µL of EGF solution
Insulin Solution (Catalog Number I9278)
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2–8 °C.
10.8 µL of EGF solution (1
Insulin Solution (Catalog Number I9278)
108 µL of Cholera toxin solution (1 mg/mL), prepared
by dissolving Catalog Number C8052 in sterile
water. Store solution at 2–8 °C.
10.8 µL of EGF solution (1