).
This IGF-1 ELISA kit shows no cross-reactivity with any
of the cytokines tested: Human Angiogenin, BDNF,
BLC, ENA-78, FGF-4, IGFBP-1, IGFBP-2, IGFBP-3,
IGFBP-4, IL-1, IL-1, IL-2, IL-3, IL
may be stored in liquid nitrogen
(LN2) for control purposes. To accomplish this, 1 x 107 - 108 cells are frozen at 1 ± 0.3°C per
minute in a medium containing 50% fetal calf serum, 40% RPMI-1640 (or
The antibody preparation does not contain pre-
servatives or stabilizers.
Affinity Ka = 1 × 108/M
Isoelectric
Point
6.4
2. How to Use this Product
2.1 Before You Begin
Epitope Tagging
HPLC.
The antibody preparation does not contain pre-
servatives or stabilizers.
Affinity Ka = 1 × 108/M
Isoelectric
Point
6.4
2. How to Use this Product
2.1 Before You Begin
Epitope Tagging
sample volume). In this case 350 µl
lysis buffer (vial 1) have to be added to get the required final volume
of 400 µl.
Number of cells 1 × 108 2 × 107 1 × 107 2 × 106 2 ×
function. J. Clin. Invest. 108: 25-30.
Nguyen M et al. (1997) The prostaglandin receptor EP4 triggers remodelling of the
cardiovascular system at birth. Nature 390: 78-81.
Yoshida K et al. (2002
cells, from 20-100 µL of packed cell
volume), or from a much larger number of cells
(108–1010 cells, from ~1 mL of packed cell volume).
Nuclear proteins can be extracted from the following
fresh
solution
1:500.
A.2 Microtubule preparation from cells 6-8
Tubulin preparation from cells requires a large amount
of cells. The procedure below is directed for at least
6 X 108 cells.
1. Add cold
number of proliferative diseases, particu-
larly in cancer. There are three Cdc25 homologs: A, B and
C [108], which are expressed and activated at different
times during the cell cycle acting on different cyclin-Cdk
10 38 39 97
3 50 41 79 90
4 100 108 129 94
Varying concentrations of GLP-1 were spiked into four human plasma samples and the GLP-1
content determined by RIA.
1990, 102, 78.
59 C. Unverzagt, et al., J. Am. Chem. Soc., 1990, 112, 9308.
60 C. Augé, et al., Carbohydr. Res., 1989, 193, 288.
61 G. Baisch, R. Öhrlein,, Angew. Chem., 1996, 108