aliquots for up to 6 months after date of receipt. Avoid
repeated freeze/thaw cycles.
1. Jacquemin E et al (1994) PNAS 91:133-137; Kullak-Ublick, GA et al (1995)
Gastroenterol. 109:1274-1282
Catalog # PCYTMAG-23K, PCYTMG-23K-13PX, PCYTMAG23PMX13BK
QC Catalog #: PCYT-6023 Lot#: PCYT-109 and PCYT-209
Note: The Quality Control Ranges are generated with overnight assay format using serum
solutions at -20°C.
Equilibrate a heating block or water bath at 65°C
Sample Preparation:
From a 6- or 12-well microtiter plate choose an
occlusion-negative well and disrupt the cell
monolayer with a sterile
Medium with 4500
mg glucose/L and one part NCTC 109. H-Y Medium also
contains oxaloacetate, sodium pyruvate, and bovine insulin.
H-Y MEDIUM [DME/NCTC 109 7:1 Mixture], Product No.
H9014 HYBRI-MAX® is
. Cytochem., 49(12),
1565-1572 (2001).
4. Zorn, U. et al., Characterization of modified low
density lipoprotein subfractions by capillary
isotachophoresis. Electrophoresis, 22(6),
1143-1149 (
Biotechnol. 56, 61-109. (1997)
11. Levine, A.D., et al., High Level Expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia
coli J. Biol. Chem. 270, 7445-7452 (1995)
12. Rudloph, R.
is stable for up to 6 months when
stored at –20 °C when purged and stored with an inert
gas such as argon or nitrogen. Aqueous solutions of
arachidonic acid should be used within 12 hours.
Although aqueous
Description
Molecular Formula: C6H6O2
Molecular Weight: 110.1
CAS Number: 108-46-3
Melting Point: 109-111 °C1
Boiling Point: 280 °C (compound will volatilize at low
temperature and is slightly volatile
molecular mass of the
monomer is 109 kDa, but as a result of glycosylation,
the recombinant monomer migrates at >150 kDa on
reducing SDS-PAGE.1,2
Oncostatin M is a member of the IL-6-type family of
cytokines
Biotechnol. 56, 61-109. (1997)
11. Levine, A.D., et al., High Level Expression and Refolding of Mouse Interleukin 4 Synthesized in Escherichia
coli J. Biol. Chem. 270, 7445-7452 (1995)
12. Rudloph, R.
Description
Antibody sensitized sheep erythrocytes (EA7S) are
supplied at a concentration of 1 × 109 cells/ml in a
volume of two milliliters per vial. The cells are
sensitized with an antibody against
Sacco, P.A., et al. (1995) J. Biol. Chem. 270: 20201.
5. Wahl, J.K., et al. (1996) J. Cell Sci. 109: 1043.
6. Islam, S., et al. (1996) J. Cell Biol. 135: 1643.
7. Lewis, J.E., et al. (1997) J. Cell
optimal working dilutions by
titration test.
References
1. Oyler, G. A., et al., J. Cell. Biol., 109, 3039 (1989).
2. Oyler, G. A., et al., Proc. Natl. Acad. Sci. USA, 88,
5247 (1991).
3. Söllner
Global Science Books,
pp. 80-88 (2009).
2. Traverso-Cori, A. et al., Arch. Biochem. Biophys.,
109(1), 173-184 (1965).
The life science business of Merck operates
J. Antibiotics, 24(6), 402-404
(1971).
10. Umezawa, H., Pure Appl. Chem., 33(1), 129-144
(1973).
11. Saino, T. et al., Chem. Pharm. Bull., 30(7),
2319-2325 (1982).
12. Billinger, E. et al
(2008).
8. Chen, G. et al., Proteomics, 3, 496-504 (2003).
9. Balabanov, S. et al., Blood, 109, 1701-1711 (2007).
VS,DS,KAA,PHC,MAM 06/19-1
2019 Sigma-Aldrich Co. LLC. All rights
McGuigan, J. Physiol. 343, 253 (1983).
6 On the temperature dependence of the Na pump in sheep Purkinje fibres. H. G. Glitsch, H. Pusch, Pflügers Arch. 402, 109
(1984).
7 Changes of intracellular sodium
is stable for up to 6 months when
stored at –20 °C when purged and stored with an inert
gas such as argon or nitrogen. Aqueous solutions of
arachidonic acid should be used within 12 hours.
Although aqueous
Maintain lyophilized material at -20°C for up to 12 months after date of receipt. After
reconstitution maintain at -20°C to -70°C in undiluted aliquots for up to 6 months. Avoid
repeated freeze/thaw cycles.